In the community of localization-based super-resolution microscopy (or called localization microscopy), it is generally believed that the emission of single molecules is so weak that an EMCCD (electron multiplying charge coupled device) camera is necessary to be used as the detector by eliminating read noise. Here we evaluate the possibility of a new kind of low light detector, scientific complementary metal-oxide-semiconductor (sCMOS) camera in localization microscopy. We demonstrate experimentally that sCMOS is capable of imaging actin bundles with FWHM diameter of 37 nm, evidencing the capability of sCMOS in localization microscopy. We further characterize the noise performance of sCMOS and find out that, with the use of a bright fluorescence probe such as d2EosFP, localization microscopy imaging is now working in the shot noise limited region.
%0 Journal Article
%1 Huang2011
%A Huang, Zhen-Li
%A Zhu, Hongyu
%A Long, Fan
%A Ma, Hongqiang
%A Qin, Lingsong
%A Liu, Yongfeng
%A Ding, Jiuping
%A Zhang, Zhihong
%A Luo, Qingming
%A Zeng, Shaoqun
%D 2011
%I The Optical Society
%J Optics Express
%K cameras detectors microscopy scmos superresolution
%N 20
%P 19156
%R 10.1364/oe.19.019156
%T Localization-based super-resolution microscopy with an sCMOS camera
%V 19
%X In the community of localization-based super-resolution microscopy (or called localization microscopy), it is generally believed that the emission of single molecules is so weak that an EMCCD (electron multiplying charge coupled device) camera is necessary to be used as the detector by eliminating read noise. Here we evaluate the possibility of a new kind of low light detector, scientific complementary metal-oxide-semiconductor (sCMOS) camera in localization microscopy. We demonstrate experimentally that sCMOS is capable of imaging actin bundles with FWHM diameter of 37 nm, evidencing the capability of sCMOS in localization microscopy. We further characterize the noise performance of sCMOS and find out that, with the use of a bright fluorescence probe such as d2EosFP, localization microscopy imaging is now working in the shot noise limited region.
@article{Huang2011,
abstract = {In the community of localization-based super-resolution microscopy (or called localization microscopy), it is generally believed that the emission of single molecules is so weak that an EMCCD (electron multiplying charge coupled device) camera is necessary to be used as the detector by eliminating read noise. Here we evaluate the possibility of a new kind of low light detector, scientific complementary metal-oxide-semiconductor (sCMOS) camera in localization microscopy. We demonstrate experimentally that sCMOS is capable of imaging actin bundles with FWHM diameter of 37 nm, evidencing the capability of sCMOS in localization microscopy. We further characterize the noise performance of sCMOS and find out that, with the use of a bright fluorescence probe such as d2EosFP, localization microscopy imaging is now working in the shot noise limited region.},
added-at = {2020-04-06T13:11:22.000+0200},
author = {Huang, Zhen-Li and Zhu, Hongyu and Long, Fan and Ma, Hongqiang and Qin, Lingsong and Liu, Yongfeng and Ding, Jiuping and Zhang, Zhihong and Luo, Qingming and Zeng, Shaoqun},
biburl = {https://www.bibsonomy.org/bibtex/290cd1b3e7b44110c578896bfe6902a47/kfriedl},
doi = {10.1364/oe.19.019156},
file = {::},
interhash = {7af5698160e0d3dd6ad3980c8bc2afa4},
intrahash = {90cd1b3e7b44110c578896bfe6902a47},
issn = {1094-4087},
journal = {Optics Express},
keywords = {cameras detectors microscopy scmos superresolution},
month = sep,
number = 20,
pages = 19156,
pmid = {21996858},
publisher = {The Optical Society},
timestamp = {2020-04-06T14:01:03.000+0200},
title = {{Localization-based super-resolution microscopy with an sCMOS camera}},
volume = 19,
year = 2011
}