%0 Journal Article %1 citeulike:693533 %A Xu, Y. %A Halsall, B. %A Heineman, W. R. %C Department of Chemistry, University of Cincinnati, OH 45221-0172. %D 1990 %J Clin Chem %K amperometry fia a-p immunoassay electrochemistry heterogeneous %N 11 %P 1941--1944 %T Heterogeneous enzyme immunoassay of alpha-fetoprotein in maternal serum by flow-injection amperometric detection of 4-aminophenol. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1700742 %V 36 %X A sandwich-type heterogeneous enzyme immunoassay with flow-injection analysis for alpha-fetoprotein (AFP) in human serum has been developed. 4-Aminophenol, the product of enzymatic reaction, is detected amperometrically. The interassay CV for this electrochemical enzyme immunoassay was less than 8.2%, with a minimum detection limit for AFP of 0.163 micrograms/L. The calibration curve had a linear range of 0.316-100 micrograms/L. Studies with 48 human maternal serum samples, comparing results by this method with those by a commercial kit, showed a good correlation (r = 0.961). This procedure provides an alternative method for determining low concentrations of AFP in human maternal serum.

@article{citeulike:693533, abstract = {A sandwich-type heterogeneous enzyme immunoassay with flow-injection analysis for alpha-fetoprotein (AFP) in human serum has been developed. 4-Aminophenol, the product of enzymatic reaction, is detected amperometrically. The interassay CV for this electrochemical enzyme immunoassay was less than 8.2%, with a minimum detection limit for AFP of 0.163 micrograms/L. The calibration curve had a linear range of 0.316-100 micrograms/L. Studies with 48 human maternal serum samples, comparing results by this method with those by a commercial kit, showed a good correlation (r = 0.961). This procedure provides an alternative method for determining low concentrations of AFP in human maternal serum.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Department of Chemistry, University of Cincinnati, OH 45221-0172.}, author = {Xu, Y. and Halsall, B. and Heineman, W. R.}, biburl = {https://www.bibsonomy.org/bibtex/29134f12813f3ad241e6eb82377387a77/biblio24}, citeulike-article-id = {693533}, interhash = {a82669ee835355636ba17c2bd2b7f205}, intrahash = {9134f12813f3ad241e6eb82377387a77}, issn = {0009-9147}, journal = {Clin Chem}, keywords = {amperometry fia a-p immunoassay electrochemistry heterogeneous}, month = {November}, number = 11, pages = {1941--1944}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Heterogeneous enzyme immunoassay of alpha-fetoprotein in maternal serum by flow-injection amperometric detection of 4-aminophenol.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=1700742}, volume = 36, year = 1990 }