Reaction mechanisms between ozone and extracellular polymeric substances (EPS) can be the key of understanding the improvements in microbial aggregates properties by low-concentration ozonation. In this study, EPS are extracted from activated sludge and treated continuously by ozone gas at 270 ± 41 ppm. The reaction between ozone and EPS was investigated by observation of EPS component concentrations, functional groups and molecular weight distributions using UV–Vis spectrometry, excitation-emission matrix fluorescence spectroscopy (EEM), high performance size-exclusion chromatography (HPSEC) and gas chromatography-mass spectrometry (GC–MS). In a 12-hour-ozonation experiment, significant ozone consumption was observed in the first 4 h and protein concentration in EPS solution was reduced by 30 ± 12%. However, the polysaccharides concentration only had a slightly decrease at the end of the ozonation process. UV–Vis spectra and EEM spectra results suggest that ozone removed protein and fluorescent matters (SMP and tryptophan-like aromatic protein) rapidly by attacking specific amino acid residues on polypeptide chain. After ozonation, the molecular weight of polysaccharide and protein dropped by 4 orders of magnitude according to HPSEC results. TOC concentration of EPS solution was reduced by 13 ± 2% after ozonation. The loss in TOC could be explained by the observation of volatile organic compounds such as carboxylic acids, aldehydes and ketones in the off-gas detected by GC–MS. The results in this study can provide a better understanding towards the mechanisms of improvements in activated sludge properties by ozonation.
%0 Journal Article
%1 meng2016degradation
%A Meng, Lu
%A Xi, Jinying
%A Yeung, Marvin
%D 2016
%J Chemosphere
%K 92c40-biochemistry-molecular-biology 92c70-microbiology biofilm
%P 248-255
%R 10.1016/j.chemosphere.2015.12.060
%T Degradation of extracellular polymeric substances (EPS) extracted from activated sludge by low-concentration ozonation
%U https://www.sciencedirect.com/science/article/pii/S0045653515305142
%V 147
%X Reaction mechanisms between ozone and extracellular polymeric substances (EPS) can be the key of understanding the improvements in microbial aggregates properties by low-concentration ozonation. In this study, EPS are extracted from activated sludge and treated continuously by ozone gas at 270 ± 41 ppm. The reaction between ozone and EPS was investigated by observation of EPS component concentrations, functional groups and molecular weight distributions using UV–Vis spectrometry, excitation-emission matrix fluorescence spectroscopy (EEM), high performance size-exclusion chromatography (HPSEC) and gas chromatography-mass spectrometry (GC–MS). In a 12-hour-ozonation experiment, significant ozone consumption was observed in the first 4 h and protein concentration in EPS solution was reduced by 30 ± 12%. However, the polysaccharides concentration only had a slightly decrease at the end of the ozonation process. UV–Vis spectra and EEM spectra results suggest that ozone removed protein and fluorescent matters (SMP and tryptophan-like aromatic protein) rapidly by attacking specific amino acid residues on polypeptide chain. After ozonation, the molecular weight of polysaccharide and protein dropped by 4 orders of magnitude according to HPSEC results. TOC concentration of EPS solution was reduced by 13 ± 2% after ozonation. The loss in TOC could be explained by the observation of volatile organic compounds such as carboxylic acids, aldehydes and ketones in the off-gas detected by GC–MS. The results in this study can provide a better understanding towards the mechanisms of improvements in activated sludge properties by ozonation.
@article{meng2016degradation,
abstract = {Reaction mechanisms between ozone and extracellular polymeric substances (EPS) can be the key of understanding the improvements in microbial aggregates properties by low-concentration ozonation. In this study, EPS are extracted from activated sludge and treated continuously by ozone gas at 270 ± 41 ppm. The reaction between ozone and EPS was investigated by observation of EPS component concentrations, functional groups and molecular weight distributions using UV–Vis spectrometry, excitation-emission matrix fluorescence spectroscopy (EEM), high performance size-exclusion chromatography (HPSEC) and gas chromatography-mass spectrometry (GC–MS). In a 12-hour-ozonation experiment, significant ozone consumption was observed in the first 4 h and protein concentration in EPS solution was reduced by 30 ± 12%. However, the polysaccharides concentration only had a slightly decrease at the end of the ozonation process. UV–Vis spectra and EEM spectra results suggest that ozone removed protein and fluorescent matters (SMP and tryptophan-like aromatic protein) rapidly by attacking specific amino acid residues on polypeptide chain. After ozonation, the molecular weight of polysaccharide and protein dropped by 4 orders of magnitude according to HPSEC results. TOC concentration of EPS solution was reduced by 13 ± 2% after ozonation. The loss in TOC could be explained by the observation of volatile organic compounds such as carboxylic acids, aldehydes and ketones in the off-gas detected by GC–MS. The results in this study can provide a better understanding towards the mechanisms of improvements in activated sludge properties by ozonation.},
added-at = {2024-02-02T04:25:58.000+0100},
author = {Meng, Lu and Xi, Jinying and Yeung, Marvin},
biburl = {https://www.bibsonomy.org/bibtex/294195009ff1e3a5a513c057139ce7d75/gdmcbain},
doi = {10.1016/j.chemosphere.2015.12.060},
interhash = {081d479c0dbe4a3e6db37173a52812ed},
intrahash = {94195009ff1e3a5a513c057139ce7d75},
issn = {0045-6535},
journal = {Chemosphere},
keywords = {92c40-biochemistry-molecular-biology 92c70-microbiology biofilm},
pages = {248-255},
timestamp = {2024-02-02T04:25:58.000+0100},
title = {Degradation of extracellular polymeric substances (EPS) extracted from activated sludge by low-concentration ozonation},
url = {https://www.sciencedirect.com/science/article/pii/S0045653515305142},
volume = 147,
year = 2016
}