Synthesis,Characterization and Study of the Molecular Docking With Biological Activity of Some Mannich Base Complexes Derived 4-Aminobenzoic Acid
CENTRAL ASIAN JOURNAL OF MEDICAL AND NATURAL SCIENCE, 4 (5):
775-785 (September 2023)Abstract
This study involves the preparation of various organic compounds. Mannich base derivatives (A1, A2) were prepared by reacting 4-aminobenazoic acid with saccharin and benzaldehyde derivatives, using absolute ethanol as a solvent. Several complexes of Mannich bases (A3-A6) were obtained by reacting equimolar amounts of Mannich base derivatives (A1, A2) with the metal salt solution of cobalt and cadmium (MCln.XH2O), using ethanol as a solvent. The synthesized compounds and complexes were characterized using various spectroscopic techniques, including Fourier-Transform Infrared (FT-IR) spectroscopy, and Nuclear Magnetic Resonance (1H,13C-NMR) spectroscopy. Additionally, their melting points, purity, molar conductivity, and magnetic susceptibility were determined. The impact of some prepared compounds and complexes on the growth of two antibiotic-resistant bacterial strains, namely the Gram-negative Pseudomonas Aeruginosa and the Gram-positive Streptococcus Mutans, was studied. Amoxicillin, Ampicillin, and Ciprofloxacin were used as a control antibiotic. Some of the synthesized compounds exhibited significant inhibitory activity against the tested bacterial strains. Molecular docking studies were conducted for the compounds (A1, A2) against Pseudomonas Aeruginosa using the MOE software (2009). The energy minimization process was employed to achieve the most stable conformation (lowest energy barrier) for these compounds.