@hymi

Banding pattern indicative of echinococcosis in a commercial cysticercosis Western blot

, , , и . European Journal of Medical Research, 14 (10): 451--452 (сентября 2009)PMID: 19748853.

Аннотация

\textlessAbstractText Label=ÖBJECTIVE" NlmCategory=ÖBJECTIVE"\textgreaterA commercial cysticercosis Western blot was evaluated for serological cross-reactivity of sera from patients with alveolar (AE) and cystic echinococcosis (CE).\textless/AbstractText\textgreater \textlessAbstractText Label="METHODS" NlmCategory="METHODS"\textgreaterA total of 161 sera were examined, including 31 sera from AE-patients, 11 sera from CE- patients, 9 sera from patients with other parasitic diseases and 109 sera from patients with unrelated medical conditions. All AE- and CE-sera were also examined by the echinococcosis Western blot.\textless/AbstractText\textgreater \textlessAbstractText Label="RESULTS" NlmCategory="RESULTS"\textgreaterMore sera from patients with AE than with CE showed cross-reactivity in the form of ladder-like patterns ("Mikado aspect") and untypical bands at 6-8 kDa (71\% and 77.4\% versus 27.3\% and 45.5\%, respectively). In contrast, triplets of bands in the area above 50 kDa and between 24 and 39-42 kDa were more frequent in CE than in AE sera. The fuzzy band at 50-55 kDa typical for cysticercosis was absent in all AE and CE sera.\textless/AbstractText\textgreater \textlessAbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS"\textgreaterAtypical banding patterns in the cysticercosis Western blot should raise the suspicion of a meta?cestode infection different from Taenia solium, i.e. Echinococcus multilocularis or E. granulosus, especially when the Mikado aspect and an altered 6-8 kDa band is visible in the absence of a fuzzy 50-55 kDa band.\textless/AbstractText\textgreater

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