%0 Journal Article %1 lorenz2015rational %A Lorenz, R. %A He, D. %A Kim, C. %A Lim, C. %A Herberg, F.W. %D 2015 %J BMC Pharmacology and Toxicology %K herberg myown %N Suppl 1 %P A64 %R 10.1186/2050-6511-16-S1-A64 %T Rational design of a PKA-based sensor for cGMP %U http://www.biomedcentral.com/2050-6511/16/S1/A64 %V 16 %X The cAMP-dependent protein kinase (PKA) and the cGMP-dependent protein kinase (PKG) are highly homologous enzymes differing in their specificity for cAMP and cGMP, respectively. Recent structure-function studies led to the identification of key residues responsible for cGMP-specificity in PKG. Introduction of these amino acids into PKA switches its cyclic nucleotide selectivity. Here we demonstrate that the same mutations turn a cAMP-specific FRET-based A-kinase activity reporter (AKAR) into a cGMP-specific reporter.

@article{lorenz2015rational, abstract = {The cAMP-dependent protein kinase (PKA) and the cGMP-dependent protein kinase (PKG) are highly homologous enzymes differing in their specificity for cAMP and cGMP, respectively. Recent structure-function studies led to the identification of key residues responsible for cGMP-specificity in PKG. Introduction of these amino acids into PKA switches its cyclic nucleotide selectivity. Here we demonstrate that the same mutations turn a cAMP-specific FRET-based A-kinase activity reporter (AKAR) into a cGMP-specific reporter. }, added-at = {2015-10-22T15:13:42.000+0200}, author = {Lorenz, R. and He, D. and Kim, C. and Lim, C. and Herberg, F.W.}, biburl = {https://www.bibsonomy.org/bibtex/215e7798c9ee8a5bf762866b72f457798/biochemie}, description = {BMC Pharmacology and Toxicology | Full text | Rational design of a PKA-based sensor for cGMP}, doi = {10.1186/2050-6511-16-S1-A64}, interhash = {29b666c38b8d91017469ff1d87ddedd0}, intrahash = {15e7798c9ee8a5bf762866b72f457798}, issn = {2050-6511}, journal = {BMC Pharmacology and Toxicology}, keywords = {herberg myown}, number = {Suppl 1}, pages = {A64}, timestamp = {2016-11-04T11:17:32.000+0100}, title = {Rational design of a PKA-based sensor for cGMP}, url = {http://www.biomedcentral.com/2050-6511/16/S1/A64}, volume = 16, year = 2015 }