%0 Journal Article %1 glenz2022major %A Glenz, R. %A Kaiping, A. %A Gopfert, D. %A Weber, H. %A Lambour, B. %A Sylvester, M. %A Froschel, C. %A Mueller, M. J. %A Osman, M. %A Waller, F. %D 2022 %J Sci Rep %K Agrobacterium myOwn tumefaciens %N 1 %P 1081 %R 10.1038/s41598-022-05083-4 %T The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens %U https://www.ncbi.nlm.nih.gov/pubmed/35058538 %V 12 %X Sphingolipid long chain bases (LCBs) are building blocks of sphingolipids and can serve as signalling molecules, but also have antimicrobial activity and were effective in reducing growth of a range of human pathogens. In plants, LCBs are linked to cell death processes and the regulation of defence reactions against pathogens, but their role in directly influencing growth of plant-interacting microorganisms has received little attention. Therefore, we tested the major plant LCB phytosphingosine in in vitro tests with the plant pathogenic fungi Verticillium longisporum, Fusarium graminearum and Sclerotinia sclerotiorum, the plant symbiotic fungal endophyte Serendipita indica, the bacterial pathogens Pseudomonas syringae pv. tomato (Pst), Agrobacterium tumefaciens, and the related beneficial strain Rhizobium radiobacter. Phytosphingosine inhibited growth of these organisms at micromolar concentrations. Among the fungal pathogens, S. sclerotiorum was the most, and F. graminearum was the least sensitive. 15.9 mug/mL phytosphingosine effectively killed 95% of the three bacterial species. Plant disease symptoms and growth of Pst were also inhibited by phytosphingosine when co-infiltrated into Arabidopsis leaves, with no visible negative effect on host tissue. Taken together, we demonstrate that the plant LCB phytosphingosine inhibits growth of plant-interacting microorganisms. We discuss the potential of elevated LCB levels to enhance plant pathogen resistance.

@article{glenz2022major, abstract = {Sphingolipid long chain bases (LCBs) are building blocks of sphingolipids and can serve as signalling molecules, but also have antimicrobial activity and were effective in reducing growth of a range of human pathogens. In plants, LCBs are linked to cell death processes and the regulation of defence reactions against pathogens, but their role in directly influencing growth of plant-interacting microorganisms has received little attention. Therefore, we tested the major plant LCB phytosphingosine in in vitro tests with the plant pathogenic fungi Verticillium longisporum, Fusarium graminearum and Sclerotinia sclerotiorum, the plant symbiotic fungal endophyte Serendipita indica, the bacterial pathogens Pseudomonas syringae pv. tomato (Pst), Agrobacterium tumefaciens, and the related beneficial strain Rhizobium radiobacter. Phytosphingosine inhibited growth of these organisms at micromolar concentrations. Among the fungal pathogens, S. sclerotiorum was the most, and F. graminearum was the least sensitive. 15.9 mug/mL phytosphingosine effectively killed 95% of the three bacterial species. Plant disease symptoms and growth of Pst were also inhibited by phytosphingosine when co-infiltrated into Arabidopsis leaves, with no visible negative effect on host tissue. Taken together, we demonstrate that the plant LCB phytosphingosine inhibits growth of plant-interacting microorganisms. We discuss the potential of elevated LCB levels to enhance plant pathogen resistance.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Glenz, R. and Kaiping, A. and Gopfert, D. and Weber, H. and Lambour, B. and Sylvester, M. and Froschel, C. and Mueller, M. J. and Osman, M. and Waller, F.}, biburl = {https://www.bibsonomy.org/bibtex/2d0e586f98ddd1a2448b2abd24d28a9c7/jvsi_all}, doi = {10.1038/s41598-022-05083-4}, interhash = {2aafeef2101995787d0f9049c8f90299}, intrahash = {d0e586f98ddd1a2448b2abd24d28a9c7}, issn = {2045-2322 (Electronic) 2045-2322 (Linking)}, journal = {Sci Rep}, keywords = {Agrobacterium myOwn tumefaciens}, note = {Glenz, Rene Kaiping, Agnes Gopfert, Delia Weber, Hannah Lambour, Benjamin Sylvester, Marvin Froschel, Christian Mueller, Martin J Osman, Mohamed Waller, Frank eng WA 2478/4-1/Deutsche Forschungsgemeinschaft/ Research Support, Non-U.S. Gov't England 2022/01/22 Sci Rep. 2022 Jan 20;12(1):1081. doi: 10.1038/s41598-022-05083-4.}, number = 1, pages = 1081, timestamp = {2024-02-15T15:08:22.000+0100}, title = {The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/35058538}, volume = 12, year = 2022 }