Alternating-laser excitation (ALEX) spectroscopy has recently been
added to the single-molecule spectroscopy toolkit. ALEX monitors
interaction and stoichiometry of biomolecules, reports on biomolecular
structure by measuring accurate Förster resonance energy transfer
(FRET) efficiencies, and allows sorting of subpopulations on the
basis of stoichiometry and FRET. Here, we demonstrate that a simple
combination of one continuous-wave donor-excitation laser and one
directly modulated acceptor-excitation laser (Periodic Acceptor eXcitation)
is sufficient to recapitulate the capabilities of ALEX while minimizing
the cost and complexity associated with use of modulation techniques.
%0 Journal Article
%1 Doose2007d
%A Doose, Sören
%A Heilemann, Mike
%A Michalet, Xavier
%A Weiss, Shimon
%A Kapanidis, Achillefs
%D 2007
%I Springer Berlin / Heidelberg
%J European Biophysics Journal
%K doose mike
%P 669-674
%T Periodic acceptor excitation spectroscopy of single molecules
%U http://dx.doi.org/10.1007/s00249-007-0133-7
%V 36
%X Alternating-laser excitation (ALEX) spectroscopy has recently been
added to the single-molecule spectroscopy toolkit. ALEX monitors
interaction and stoichiometry of biomolecules, reports on biomolecular
structure by measuring accurate Förster resonance energy transfer
(FRET) efficiencies, and allows sorting of subpopulations on the
basis of stoichiometry and FRET. Here, we demonstrate that a simple
combination of one continuous-wave donor-excitation laser and one
directly modulated acceptor-excitation laser (Periodic Acceptor eXcitation)
is sufficient to recapitulate the capabilities of ALEX while minimizing
the cost and complexity associated with use of modulation techniques.
@article{Doose2007d,
abstract = {Alternating-laser excitation (ALEX) spectroscopy has recently been
added to the single-molecule spectroscopy toolkit. ALEX monitors
interaction and stoichiometry of biomolecules, reports on biomolecular
structure by measuring accurate Förster resonance energy transfer
(FRET) efficiencies, and allows sorting of subpopulations on the
basis of stoichiometry and FRET. Here, we demonstrate that a simple
combination of one continuous-wave donor-excitation laser and one
directly modulated acceptor-excitation laser (Periodic Acceptor eXcitation)
is sufficient to recapitulate the capabilities of ALEX while minimizing
the cost and complexity associated with use of modulation techniques.},
added-at = {2011-03-09T13:59:57.000+0100},
affiliation = {Oxford University Clarendon Laboratory, Department of Physics and
IRC in Bionanotechnology Oxford UK},
author = {Doose, Sören and Heilemann, Mike and Michalet, Xavier and Weiss, Shimon and Kapanidis, Achillefs},
biburl = {https://www.bibsonomy.org/bibtex/2bad619c6a8f97a07758242daa1567613/reichert},
interhash = {9dd88c65024e6967998eebf90981ff2f},
intrahash = {bad619c6a8f97a07758242daa1567613},
issn = {0175-7571},
issue = {6},
journal = {European Biophysics Journal},
keyword = {Physics and Astronomy},
keywords = {doose mike},
note = {10.1007/s00249-007-0133-7},
owner = {biot057},
pages = {669-674},
publisher = {Springer Berlin / Heidelberg},
timestamp = {2011-03-09T13:59:57.000+0100},
title = {Periodic acceptor excitation spectroscopy of single molecules},
url = {http://dx.doi.org/10.1007/s00249-007-0133-7},
volume = 36,
year = 2007
}