%0 Journal Article %1 Benovic1991 %A Benovic, J. L. %A Onorato, J. J. %A Arriza, J. L. %A Stone, W. C. %A Lohse, M. %A Jenkins, N. A. %A Gilbert, D. J. %A Copeland, N. G. %A Caron, M. G. %A Lefkowitz, R. J. %D 1991 %J J Biol Chem %K *Chromosome *Cyclic 3 AMP-Dependent Acid Amino Animals Base Blotting, Cloning, Cricetinae DNA/genetics Data G-Protein-Coupled Kinase Kinases Kinases/*genetics/metabolism Mapping Messenger/genetics/metabolism Mice Molecular Northern Protein RNA, Receptor Sequence beta-Adrenergic %N 23 %P 14939-46 %T Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1869533 %V 266 %X The beta-adrenergic receptor kinase (beta ARK) specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Structural features of this enzyme have been elucidated recently by the isolation of a cDNA that encodes bovine beta ARK. Utilizing a catalytic domain fragment of the beta ARK cDNA to screen a bovine brain cDNA library we have isolated a clone encoding a beta ARK-related enzyme which we have termed beta ARK2. Overall, this enzyme has 85% amino acid identity with beta ARK, with the protein kinase catalytic domain having 95% identity. The ability of beta ARK2 to phosphorylate various substrates was studied after expression in COS 7 cells. Although beta ARK2 is essentially equiactive with beta ARK in phosphorylating an acid-rich synthetic model peptide it was only approximately 50% as active when the substrate was the agonist-occupied beta 2-adrenergic receptor and only approximately 20% as active toward light-bleached rhodopsin. As with beta ARK, phosphorylation of the receptor substrates by beta ARK2 was completely stimulus dependent. RNA blot analysis with selected bovine tissues reveals an mRNA of 8 kilobases with a distribution similar to that of beta ARK. More detailed RNA analysis using a ribonuclease protection assay in various rat tissues suggests that the beta ARK2 message is present at much lower levels (typically 10-20%) than the beta ARK message. In the rat the beta ARK2 mRNA is localized predominantly in neuronal tissues although low levels are also observed in various peripheral tissues. The beta ARK2 gene has been localized to a region of mouse chromosome 5 whereas the beta ARK gene is localized on mouse chromosome 19. These data suggest the existence of a "family" of receptor kinases which may serve broadly to regulate receptor function.

@article{Benovic1991, abstract = {The beta-adrenergic receptor kinase (beta ARK) specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Structural features of this enzyme have been elucidated recently by the isolation of a cDNA that encodes bovine beta ARK. Utilizing a catalytic domain fragment of the beta ARK cDNA to screen a bovine brain cDNA library we have isolated a clone encoding a beta ARK-related enzyme which we have termed beta ARK2. Overall, this enzyme has 85% amino acid identity with beta ARK, with the protein kinase catalytic domain having 95% identity. The ability of beta ARK2 to phosphorylate various substrates was studied after expression in COS 7 cells. Although beta ARK2 is essentially equiactive with beta ARK in phosphorylating an acid-rich synthetic model peptide it was only approximately 50% as active when the substrate was the agonist-occupied beta 2-adrenergic receptor and only approximately 20% as active toward light-bleached rhodopsin. As with beta ARK, phosphorylation of the receptor substrates by beta ARK2 was completely stimulus dependent. RNA blot analysis with selected bovine tissues reveals an mRNA of 8 kilobases with a distribution similar to that of beta ARK. More detailed RNA analysis using a ribonuclease protection assay in various rat tissues suggests that the beta ARK2 message is present at much lower levels (typically 10-20%) than the beta ARK message. In the rat the beta ARK2 mRNA is localized predominantly in neuronal tissues although low levels are also observed in various peripheral tissues. The beta ARK2 gene has been localized to a region of mouse chromosome 5 whereas the beta ARK gene is localized on mouse chromosome 19. These data suggest the existence of a "family" of receptor kinases which may serve broadly to regulate receptor function.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Benovic, J. L. and Onorato, J. J. and Arriza, J. L. and Stone, W. C. and Lohse, M. and Jenkins, N. A. and Gilbert, D. J. and Copeland, N. G. and Caron, M. G. and Lefkowitz, R. J.}, biburl = {https://www.bibsonomy.org/bibtex/2bdfe405b66c353d3c75d038ebc1f3a48/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {72dc3d2c22deabd780769e40fe42e8f1}, intrahash = {bdfe405b66c353d3c75d038ebc1f3a48}, issn = {0021-9258 (Print) 0021-9258 (Linking)}, journal = {J Biol Chem}, keywords = {*Chromosome *Cyclic 3 AMP-Dependent Acid Amino Animals Base Blotting, Cloning, Cricetinae DNA/genetics Data G-Protein-Coupled Kinase Kinases Kinases/*genetics/metabolism Mapping Messenger/genetics/metabolism Mice Molecular Northern Protein RNA, Receptor Sequence beta-Adrenergic}, month = {Aug 15}, note = {Benovic, J L Onorato, J J Arriza, J L Stone, W C Lohse, M Jenkins, N A Gilbert, D J Copeland, N G Caron, M G Lefkowitz, R J GM44944/GM/NIGMS NIH HHS/United States HL16037/HL/NHLBI NIH HHS/United States N01-CO-74101/CO/NCI NIH HHS/United States Research Support, U.S. Gov't, P.H.S. United states The Journal of biological chemistry J Biol Chem. 1991 Aug 15;266(23):14939-46.}, number = 23, pages = {14939-46}, shorttitle = {Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family}, timestamp = {2010-12-14T18:22:02.000+0100}, title = {Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=1869533}, volume = 266, year = 1991 }