Real-time monitoring of somatostatin receptor-cAMP signaling in live
pituitary
S. Jacobs, D. Calebiro, V. Nikolaev, M. Lohse, and S. Schulz. Endocrinology, 151 (9):
4560-5(September 2010)Jacobs, Stefan Calebiro, Davide Nikolaev, Viacheslav O Lohse, Martin
J Schulz, Stefan United States Endocrinology Endocrinology. 2010
Sep;151(9):4560-5. Epub 2010 Jul 7..
Abstract
Fluorescence resonance energy transfer using genetically encoded biosensors
has proven to be a powerful technique to monitor the spatiotemporal
dynamics of cAMP signals stimulated by G(s)-coupled receptors in
living cells. In contrast, real-time imaging of G(i)-mediated cAMP
signals under native conditions remains challenging. Here, we describe
the use of transgenic mice for cAMP imaging in living pituitary slices
and primary pituitary cells. This technique can be widely used to
assess the contribution of various pituitary receptors, including
individual G(i) protein-coupled somatostatin receptors, to the regulation
of cAMP levels under physiologically relevant settings.
Jacobs, Stefan Calebiro, Davide Nikolaev, Viacheslav O Lohse, Martin
J Schulz, Stefan United States Endocrinology Endocrinology. 2010
Sep;151(9):4560-5. Epub 2010 Jul 7.
%0 Journal Article
%1 Jacobs2010
%A Jacobs, S.
%A Calebiro, D.
%A Nikolaev, V. O.
%A Lohse, M. J.
%A Schulz, S.
%D 2010
%J Endocrinology
%K AMP/*metabolism Animals Cultured Cyclic Dynamics Energy Exchange Factors Factors/genetics/metabolism Female Fluorescence G-Protein-Coupled/genetics/metabolism Gland/cytology/*metabolism Guanine Knockout Luminescent Male Mice Molecular Nucleotide Pituitary Proteins/genetics/metabolism Resonance Signal Simulation Somatostatin/genetics/*metabolism Time Transduction Transfer/methods Transgenic Receptor Cell
%N 9
%P 4560-5
%T Real-time monitoring of somatostatin receptor-cAMP signaling in live
pituitary
%U http://www.ncbi.nlm.nih.gov/pubmed/20610560
%V 151
%X Fluorescence resonance energy transfer using genetically encoded biosensors
has proven to be a powerful technique to monitor the spatiotemporal
dynamics of cAMP signals stimulated by G(s)-coupled receptors in
living cells. In contrast, real-time imaging of G(i)-mediated cAMP
signals under native conditions remains challenging. Here, we describe
the use of transgenic mice for cAMP imaging in living pituitary slices
and primary pituitary cells. This technique can be widely used to
assess the contribution of various pituitary receptors, including
individual G(i) protein-coupled somatostatin receptors, to the regulation
of cAMP levels under physiologically relevant settings.
@article{Jacobs2010,
abstract = {Fluorescence resonance energy transfer using genetically encoded biosensors
has proven to be a powerful technique to monitor the spatiotemporal
dynamics of cAMP signals stimulated by G(s)-coupled receptors in
living cells. In contrast, real-time imaging of G(i)-mediated cAMP
signals under native conditions remains challenging. Here, we describe
the use of transgenic mice for cAMP imaging in living pituitary slices
and primary pituitary cells. This technique can be widely used to
assess the contribution of various pituitary receptors, including
individual G(i) protein-coupled somatostatin receptors, to the regulation
of cAMP levels under physiologically relevant settings.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Jacobs, S. and Calebiro, D. and Nikolaev, V. O. and Lohse, M. J. and Schulz, S.},
biburl = {https://www.bibsonomy.org/bibtex/2c05e24909b3038fe5f8dedffa6e691c7/pharmawuerz},
endnotereftype = {Journal Article},
groups = {private},
interhash = {a21af23cb50500e8ca65cb55e3eac601},
intrahash = {c05e24909b3038fe5f8dedffa6e691c7},
issn = {1945-7170 (Electronic) 0013-7227 (Linking)},
journal = {Endocrinology},
keywords = {AMP/*metabolism Animals Cultured Cyclic Dynamics Energy Exchange Factors Factors/genetics/metabolism Female Fluorescence G-Protein-Coupled/genetics/metabolism Gland/cytology/*metabolism Guanine Knockout Luminescent Male Mice Molecular Nucleotide Pituitary Proteins/genetics/metabolism Resonance Signal Simulation Somatostatin/genetics/*metabolism Time Transduction Transfer/methods Transgenic Receptor Cell},
month = Sep,
note = {Jacobs, Stefan Calebiro, Davide Nikolaev, Viacheslav O Lohse, Martin
J Schulz, Stefan United States Endocrinology Endocrinology. 2010
Sep;151(9):4560-5. Epub 2010 Jul 7.},
number = 9,
pages = {4560-5},
shorttitle = {Real-time monitoring of somatostatin receptor-cAMP signaling in live
pituitary},
timestamp = {2010-12-14T18:21:25.000+0100},
title = {Real-time monitoring of somatostatin receptor-cAMP signaling in live
pituitary},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20610560},
volume = 151,
year = 2010
}