%0 Journal Article %1 Bai:2010:Science:20133571 %A Bai, F %A Branch, R W %A Nicolau, D V %A Pilizota, T %A Steel, B C %A Maini, P K %A Berry, R M %D 2010 %J Science %K flagella structure %N 5966 %P 685-689 %R 10.1126/science.1182105 %T Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch %U http://www.ncbi.nlm.nih.gov/pubmed/20133571?dopt=Abstract %V 327 %X The bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis has a highly cooperative response. This has previously been understood in terms of the classic two-state, concerted model of allosteric regulation. Here, we used high-resolution optical microscopy to observe switching of single motors and uncover the stochastic multistate nature of the switch. Our observations are in detailed quantitative agreement with a recent general model of allosteric cooperativity that exhibits conformational spread-the stochastic growth and shrinkage of domains of adjacent subunits sharing a particular conformational state. We expect that conformational spread will be important in explaining cooperativity in other large signaling complexes.

@article{Bai:2010:Science:20133571, abstract = {The bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis has a highly cooperative response. This has previously been understood in terms of the classic two-state, concerted model of allosteric regulation. Here, we used high-resolution optical microscopy to observe switching of single motors and uncover the stochastic multistate nature of the switch. Our observations are in detailed quantitative agreement with a recent general model of allosteric cooperativity that exhibits conformational spread-the stochastic growth and shrinkage of domains of adjacent subunits sharing a particular conformational state. We expect that conformational spread will be important in explaining cooperativity in other large signaling complexes.}, added-at = {2010-02-08T10:37:36.000+0100}, author = {Bai, F and Branch, R W and Nicolau, D V and Pilizota, T and Steel, B C and Maini, P K and Berry, R M}, biburl = {https://www.bibsonomy.org/bibtex/2c18606ee91b97bd236712921e49a35f3/penkib}, description = {Conformational spread as a mechanism for cooperati... [Science. 2010] - PubMed result}, doi = {10.1126/science.1182105}, interhash = {db55ebefac6ee526b912f4dd79b1daa1}, intrahash = {c18606ee91b97bd236712921e49a35f3}, journal = {Science}, keywords = {flagella structure}, month = Feb, number = 5966, pages = {685-689}, pmid = {20133571}, timestamp = {2010-02-08T10:37:36.000+0100}, title = {Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20133571?dopt=Abstract}, volume = 327, year = 2010 }