Raman microspectroscopy was applied for an in situ localization of the malaria pigment hemozoin in Plasmodium falciparum-infected erythrocytes. The Raman spectra (lambdaexc=633 nm) of hemozoin show very intense signals with a very good signal-to-noise ratio. These in situ Raman signals of hemozoin were compared to Raman spectra of extracted hemozoin, of the synthetic analogue beta-hematin, and of hematin and hemin. beta-Hematin was synthesized according to the acid-catalyzed dehydration of hematin and the anhydrous dehydrohalogenation of hemin which lead to good crystals with lengths of about 5-30 microm. The Raman spectra (lambdaexc=1064 nm) of hemozoin and beta-hematin show almost identical behaviors, while some low wavenumber modes might be used to distinguish between the morphology of differently synthesized beta-hematin samples. The intensity pattern of the resonance Raman spectra (lambdaexc=568 nm) of hemozoin and beta-hematin differ significantly from those of hematin and hemin.
%0 Journal Article
%1 Frosch.20070920
%A Frosch, Torsten
%A Koncarevic, Sasa
%A Zedler, Linda
%A Schmitt, Michael
%A Schenzel, Karla
%A Becker, Katja
%A Popp, J�rgen
%D 2007/09/20/
%J J Phys Chem B
%K Animals Erythrocytes Hemeproteins Humans IFZ Malaria Molecular_Structure Plasmodium_falciparum Raman Spectrum_Analysis Trophozoites
%N 37
%P 11047-11056
%T In situ localization and structural analysis of the malaria pigment hemozoin
%V 111
%X Raman microspectroscopy was applied for an in situ localization of the malaria pigment hemozoin in Plasmodium falciparum-infected erythrocytes. The Raman spectra (lambdaexc=633 nm) of hemozoin show very intense signals with a very good signal-to-noise ratio. These in situ Raman signals of hemozoin were compared to Raman spectra of extracted hemozoin, of the synthetic analogue beta-hematin, and of hematin and hemin. beta-Hematin was synthesized according to the acid-catalyzed dehydration of hematin and the anhydrous dehydrohalogenation of hemin which lead to good crystals with lengths of about 5-30 microm. The Raman spectra (lambdaexc=1064 nm) of hemozoin and beta-hematin show almost identical behaviors, while some low wavenumber modes might be used to distinguish between the morphology of differently synthesized beta-hematin samples. The intensity pattern of the resonance Raman spectra (lambdaexc=568 nm) of hemozoin and beta-hematin differ significantly from those of hematin and hemin.
@article{Frosch.20070920,
abstract = {Raman microspectroscopy was applied for an in situ localization of the malaria pigment hemozoin in Plasmodium falciparum-infected erythrocytes. The Raman spectra (lambdaexc=633 nm) of hemozoin show very intense signals with a very good signal-to-noise ratio. These in situ Raman signals of hemozoin were compared to Raman spectra of extracted hemozoin, of the synthetic analogue beta-hematin, and of hematin and hemin. beta-Hematin was synthesized according to the acid-catalyzed dehydration of hematin and the anhydrous dehydrohalogenation of hemin which lead to good crystals with lengths of about 5-30 microm. The Raman spectra (lambdaexc=1064 nm) of hemozoin and beta-hematin show almost identical behaviors, while some low wavenumber modes might be used to distinguish between the morphology of differently synthesized beta-hematin samples. The intensity pattern of the resonance Raman spectra (lambdaexc=568 nm) of hemozoin and beta-hematin differ significantly from those of hematin and hemin.},
added-at = {2008-10-14T16:07:18.000+0200},
author = {Frosch, Torsten and Koncarevic, Sasa and Zedler, Linda and Schmitt, Michael and Schenzel, Karla and Becker, Katja and Popp, J�rgen},
biburl = {https://www.bibsonomy.org/bibtex/2ca1c6a601bdcd01ec74326eee3a78393/nutribiochem},
interhash = {9b5f64ac3d03bbcedf196bf3b99af632},
intrahash = {ca1c6a601bdcd01ec74326eee3a78393},
issn = {1520-6106},
journal = {J Phys Chem B},
keywords = {Animals Erythrocytes Hemeproteins Humans IFZ Malaria Molecular_Structure Plasmodium_falciparum Raman Spectrum_Analysis Trophozoites},
number = 37,
pages = {11047-11056},
timestamp = {2008-10-14T16:08:41.000+0200},
title = {In situ localization and structural analysis of the malaria pigment hemozoin},
volume = 111,
year = {2007/09/20/}
}