PISEMO, a separated local field experiment that can be performed with either direct (15)N (or (13)C) detection or indirect (1)H detection, is demonstrated on a single crystal of a model peptide. The (1)H signals modulated by (1)H-(15)N heteronuclear dipole-dipole couplings are observed stroboscopically in the windows of the multiple-pulse sequence used to attenuate (1)H-(1)H homonuclear dipole-dipole couplings. (1)H-detection yields spectra with about 2.5 times the signal to noise ratio observed with (15)N-detection under equivalent conditions. Resolution in both the (15)N chemical shift and (1)H-(15)N heteronuclear dipole-dipole coupling dimensions is similar to that observed with PISEMA, however, since only on-resonance pulses are utilized, the bandwidth is better.
%0 Journal Article
%1 wu_proton-detected_2008
%A Wu, Chin H
%A Opella, Stanley J
%D 2008
%J J. Magn. Reson.
%K Carbon Isotopes,Magnetic Isotopes,Peptides,Protons Resonance Spectroscopy,Nitrogen
%N 1
%P 165--170
%R 10.1016/j.jmr.2007.10.002
%T Proton-detected separated local field spectroscopy
%V 190
%X PISEMO, a separated local field experiment that can be performed with either direct (15)N (or (13)C) detection or indirect (1)H detection, is demonstrated on a single crystal of a model peptide. The (1)H signals modulated by (1)H-(15)N heteronuclear dipole-dipole couplings are observed stroboscopically in the windows of the multiple-pulse sequence used to attenuate (1)H-(1)H homonuclear dipole-dipole couplings. (1)H-detection yields spectra with about 2.5 times the signal to noise ratio observed with (15)N-detection under equivalent conditions. Resolution in both the (15)N chemical shift and (1)H-(15)N heteronuclear dipole-dipole coupling dimensions is similar to that observed with PISEMA, however, since only on-resonance pulses are utilized, the bandwidth is better.
@article{wu_proton-detected_2008,
abstract = {PISEMO, a separated local field experiment that can be performed with either direct (15)N (or (13)C) detection or indirect (1)H detection, is demonstrated on a single crystal of a model peptide. The (1)H signals modulated by (1)H-(15)N heteronuclear dipole-dipole couplings are observed stroboscopically in the windows of the multiple-pulse sequence used to attenuate (1)H-(1)H homonuclear dipole-dipole couplings. (1)H-detection yields spectra with about 2.5 times the signal to noise ratio observed with (15)N-detection under equivalent conditions. Resolution in both the (15)N chemical shift and (1)H-(15)N heteronuclear dipole-dipole coupling dimensions is similar to that observed with PISEMA, however, since only on-resonance pulses are utilized, the bandwidth is better.},
added-at = {2017-03-14T02:48:56.000+0100},
author = {Wu, Chin H and Opella, Stanley J},
biburl = {https://www.bibsonomy.org/bibtex/2cafbe33da97cb6f199780d8f07b8cbb8/nmrresource},
doi = {10.1016/j.jmr.2007.10.002},
interhash = {3aa71a1e98bf705c6fbd56f7431ce1f6},
intrahash = {cafbe33da97cb6f199780d8f07b8cbb8},
issn = {1090-7807},
journal = {J. Magn. Reson.},
keywords = {Carbon Isotopes,Magnetic Isotopes,Peptides,Protons Resonance Spectroscopy,Nitrogen},
month = jan,
number = 1,
pages = {165--170},
pmid = {17981481},
timestamp = {2017-03-14T02:49:21.000+0100},
title = {{Proton-detected separated local field spectroscopy}},
volume = 190,
year = 2008
}