The electrophoretic mobilities and diffusion coefficients of single-
and double-stranded DNA molecules up to 50 000 bases or base pairs
in size have been analyzed, using mobilities and diffusion coefficients
either measured by capillary electrophoresis or taken from the literature.
The Einstein equation suggests that the electrophoretic mobilities
(mu) and diffusion coefficients (D) should be related by the xpression
mu/D = Q/k(B)T, where Q is the charge of the polyion (Q = ze(o),
where z is the number of charged residues and e(o) is the fundamental
electronic charge), k(B) is Boltzmann's constant, and T is the absolute
temperature. If this equation were true, the ratio mu/zD should be
a constant equal to e(o)/k(B)T (39.6 V-1) at 20 degreesC. However,
the ratio mu/zD decreases with an increase in molecular weight for
both single-and double-stranded DNAs. The mobilities and diffusion
coefficients are better described by the modified Einstein equation
mu/(ND)-D-m = e(o)/k(B)T, where N is the number of repeat units (bases
or base pairs) in the DNA and m is a constant equal to the power
law dependence of the diffusion coefficients on molecular weight.
The average value of the ratio mu/(ND)-D-m is 40 +/- 4 V-1 for 36
single- and double-stranded DNA molecules of different sizes, close
to the theoretically expected value. The generality of the modified
Einstein equation is demonstrated by analyzing literature values
for sodium polystyrenesulfonate (PSS). The average value of the ratio
mu/(ND)-D-m is 35 +/- 6 V-1 for 14 PSS samples containing up to 855
monomers.
%0 Journal Article
%1 stellwagen03a
%A Stellwagen, E.
%A Lu, Y. J.
%A Stellwagen, N. C.
%D 2003
%J Biochemistry
%K CAPILLARY-ELECTROPHORESIS; DIFFUSION; DYNAMIC FREE IONIC-STRENGTH; LENGTH LIGHT-SCATTERING; MOBILITY; MODEL NUCLEIC-ACIDS; PERSISTENCE PHOTON-CORRELATION POLYELECTROLYTE; SINGLE-STRANDED-DNA; SOLUTION SPECTROSCOPY; TRANSLATIONAL
%N 40
%P 11745--11750
%T Unified description of electrophoresis and diffusion for DNA and
other polyions
%V 42
%X The electrophoretic mobilities and diffusion coefficients of single-
and double-stranded DNA molecules up to 50 000 bases or base pairs
in size have been analyzed, using mobilities and diffusion coefficients
either measured by capillary electrophoresis or taken from the literature.
The Einstein equation suggests that the electrophoretic mobilities
(mu) and diffusion coefficients (D) should be related by the xpression
mu/D = Q/k(B)T, where Q is the charge of the polyion (Q = ze(o),
where z is the number of charged residues and e(o) is the fundamental
electronic charge), k(B) is Boltzmann's constant, and T is the absolute
temperature. If this equation were true, the ratio mu/zD should be
a constant equal to e(o)/k(B)T (39.6 V-1) at 20 degreesC. However,
the ratio mu/zD decreases with an increase in molecular weight for
both single-and double-stranded DNAs. The mobilities and diffusion
coefficients are better described by the modified Einstein equation
mu/(ND)-D-m = e(o)/k(B)T, where N is the number of repeat units (bases
or base pairs) in the DNA and m is a constant equal to the power
law dependence of the diffusion coefficients on molecular weight.
The average value of the ratio mu/(ND)-D-m is 40 +/- 4 V-1 for 36
single- and double-stranded DNA molecules of different sizes, close
to the theoretically expected value. The generality of the modified
Einstein equation is demonstrated by analyzing literature values
for sodium polystyrenesulfonate (PSS). The average value of the ratio
mu/(ND)-D-m is 35 +/- 6 V-1 for 14 PSS samples containing up to 855
monomers.
@article{stellwagen03a,
abstract = {The electrophoretic mobilities and diffusion coefficients of single-
and double-stranded DNA molecules up to 50 000 bases or base pairs
in size have been analyzed, using mobilities and diffusion coefficients
either measured by capillary electrophoresis or taken from the literature.
The Einstein equation suggests that the electrophoretic mobilities
(mu) and diffusion coefficients (D) should be related by the xpression
mu/D = Q/k(B)T, where Q is the charge of the polyion (Q = ze(o),
where z is the number of charged residues and e(o) is the fundamental
electronic charge), k(B) is Boltzmann's constant, and T is the absolute
temperature. If this equation were true, the ratio mu/zD should be
a constant equal to e(o)/k(B)T (39.6 V-1) at 20 degreesC. However,
the ratio mu/zD decreases with an increase in molecular weight for
both single-and double-stranded DNAs. The mobilities and diffusion
coefficients are better described by the modified Einstein equation
mu/(ND)-D-m = e(o)/k(B)T, where N is the number of repeat units (bases
or base pairs) in the DNA and m is a constant equal to the power
law dependence of the diffusion coefficients on molecular weight.
The average value of the ratio mu/(ND)-D-m is 40 +/- 4 V-1 for 36
single- and double-stranded DNA molecules of different sizes, close
to the theoretically expected value. The generality of the modified
Einstein equation is demonstrated by analyzing literature values
for sodium polystyrenesulfonate (PSS). The average value of the ratio
mu/(ND)-D-m is 35 +/- 6 V-1 for 14 PSS samples containing up to 855
monomers.},
added-at = {2007-06-15T17:33:15.000+0200},
author = {Stellwagen, E. and Lu, Y. J. and Stellwagen, N. C.},
biburl = {https://www.bibsonomy.org/bibtex/2cd4030445426a87d82b941be106a344d/kaigrass},
interhash = {e2f769604d2090ee1dd7eba8d2ea3bbd},
intrahash = {cd4030445426a87d82b941be106a344d},
journal = {Biochemistry},
keywords = {CAPILLARY-ELECTROPHORESIS; DIFFUSION; DYNAMIC FREE IONIC-STRENGTH; LENGTH LIGHT-SCATTERING; MOBILITY; MODEL NUCLEIC-ACIDS; PERSISTENCE PHOTON-CORRELATION POLYELECTROLYTE; SINGLE-STRANDED-DNA; SOLUTION SPECTROSCOPY; TRANSLATIONAL},
month = {October},
number = 40,
owner = {grass},
pages = {11745--11750},
sn = {0006-2960},
timestamp = {2007-06-15T17:33:24.000+0200},
title = {Unified description of electrophoresis and diffusion for DNA and
other polyions},
ut = {ISI:000185814800021},
volume = 42,
year = 2003
}