The use of chlorophyll fluorescence to monitor photosynthetic performance in algae and plants is now widespread. This review examines how fluorescence parameters can be used to evaluate changes in photosystem II (PSII) photochemistry, linear electron flux, and CO(2) assimilation in vivo, and outlines the theoretical bases for the use of specific fluorescence parameters. Although fluorescence parameters can be measured easily, many potential problems may arise when they are applied to predict changes in photosynthetic performance. In particular, consideration is given to problems associated with accurate estimation of the PSII operating efficiency measured by fluorescence and its relationship with the rates of linear electron flux and CO(2) assimilation. The roles of photochemical and nonphotochemical quenching in the determination of changes in PSII operating efficiency are examined. Finally, applications of fluorescence imaging to studies of photosynthetic heterogeneity and the rapid screening of large numbers of plants for perturbations in photosynthesis and associated metabolism are considered.
Description
Chlorophyll fluorescence: a probe of pho... [Annu Rev Plant Biol. 2008] - PubMed - NCBI
%0 Journal Article
%1 Baker:2008:Annu-Rev-Plant-Biol:18444897
%A Baker, N R
%D 2008
%J Annu Rev Plant Biol
%K chlorophyll flourescence in_vivo non-invasive phenotyping photosynthesis
%P 89-113
%R 10.1146/annurev.arplant.59.032607.092759
%T Chlorophyll fluorescence: a probe of photosynthesis in vivo
%U http://www.ncbi.nlm.nih.gov/pubmed/18444897
%V 59
%X The use of chlorophyll fluorescence to monitor photosynthetic performance in algae and plants is now widespread. This review examines how fluorescence parameters can be used to evaluate changes in photosystem II (PSII) photochemistry, linear electron flux, and CO(2) assimilation in vivo, and outlines the theoretical bases for the use of specific fluorescence parameters. Although fluorescence parameters can be measured easily, many potential problems may arise when they are applied to predict changes in photosynthetic performance. In particular, consideration is given to problems associated with accurate estimation of the PSII operating efficiency measured by fluorescence and its relationship with the rates of linear electron flux and CO(2) assimilation. The roles of photochemical and nonphotochemical quenching in the determination of changes in PSII operating efficiency are examined. Finally, applications of fluorescence imaging to studies of photosynthetic heterogeneity and the rapid screening of large numbers of plants for perturbations in photosynthesis and associated metabolism are considered.
@article{Baker:2008:Annu-Rev-Plant-Biol:18444897,
abstract = {The use of chlorophyll fluorescence to monitor photosynthetic performance in algae and plants is now widespread. This review examines how fluorescence parameters can be used to evaluate changes in photosystem II (PSII) photochemistry, linear electron flux, and CO(2) assimilation in vivo, and outlines the theoretical bases for the use of specific fluorescence parameters. Although fluorescence parameters can be measured easily, many potential problems may arise when they are applied to predict changes in photosynthetic performance. In particular, consideration is given to problems associated with accurate estimation of the PSII operating efficiency measured by fluorescence and its relationship with the rates of linear electron flux and CO(2) assimilation. The roles of photochemical and nonphotochemical quenching in the determination of changes in PSII operating efficiency are examined. Finally, applications of fluorescence imaging to studies of photosynthetic heterogeneity and the rapid screening of large numbers of plants for perturbations in photosynthesis and associated metabolism are considered.},
added-at = {2012-03-13T12:36:48.000+0100},
author = {Baker, N R},
biburl = {https://www.bibsonomy.org/bibtex/2cdedd76fef07835641fb514313abf80f/schmidt2},
description = {Chlorophyll fluorescence: a probe of pho... [Annu Rev Plant Biol. 2008] - PubMed - NCBI},
doi = {10.1146/annurev.arplant.59.032607.092759},
interhash = {8da946c0f09f7ed813eb4fb109967857},
intrahash = {cdedd76fef07835641fb514313abf80f},
journal = {Annu Rev Plant Biol},
keywords = {chlorophyll flourescence in_vivo non-invasive phenotyping photosynthesis},
pages = {89-113},
pmid = {18444897},
timestamp = {2012-03-13T12:36:48.000+0100},
title = {Chlorophyll fluorescence: a probe of photosynthesis in vivo},
url = {http://www.ncbi.nlm.nih.gov/pubmed/18444897},
volume = 59,
year = 2008
}