An amperometric immunosensor for the detection of the lactate dehydrogenase isoenzyme LD-1 has been developed. Polyclonal antibodies for LD-1 have been covalently immobilised onto a preactivated Immunodyne ABC membrane, reacted with standard LD-1 solutions and placed onto a platinum working electrode polarised at +600 mV vs. Ag/AgCl. Lactate dehydrogenase activity has been measured by detection of the oxidation of NADH at the electrode surface. A calibration curve for LDH in the 0.005-0.12 U range has been obtained with a repeatability of 3% and a reproducibility of 10%. The probe exhibited good selectivity (response of LD-2 was 18% of LD-1) which was further improved using thermal treatment of the membranes. Measurement of LD-1 content in human control serum with the developed procedure gave a LD-1 concentration in the reported assigned range.
%0 Journal Article
%1 citeulike:470524
%A Kelly, S.
%A Compagnone, D.
%A Guilbault, G.
%C Chemistry Department, University College Cork, Ireland.
%D 1998
%J Biosens Bioelectron
%K amperometry lactate_dehydrogenase immunoassay immunosensor ag_detection electrochemistry immunoelectrode
%N 2
%P 173--179
%R 10.1016/S0956-5663(97)00105-X
%T Amperometric immunosensor for lactate dehydrogenase LD-1.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9597734
%V 13
%X An amperometric immunosensor for the detection of the lactate dehydrogenase isoenzyme LD-1 has been developed. Polyclonal antibodies for LD-1 have been covalently immobilised onto a preactivated Immunodyne ABC membrane, reacted with standard LD-1 solutions and placed onto a platinum working electrode polarised at +600 mV vs. Ag/AgCl. Lactate dehydrogenase activity has been measured by detection of the oxidation of NADH at the electrode surface. A calibration curve for LDH in the 0.005-0.12 U range has been obtained with a repeatability of 3% and a reproducibility of 10%. The probe exhibited good selectivity (response of LD-2 was 18% of LD-1) which was further improved using thermal treatment of the membranes. Measurement of LD-1 content in human control serum with the developed procedure gave a LD-1 concentration in the reported assigned range.
@article{citeulike:470524,
abstract = {An amperometric immunosensor for the detection of the lactate dehydrogenase isoenzyme LD-1 has been developed. Polyclonal antibodies for LD-1 have been covalently immobilised onto a preactivated Immunodyne ABC membrane, reacted with standard LD-1 solutions and placed onto a platinum working electrode polarised at +600 mV vs. Ag/AgCl. Lactate dehydrogenase activity has been measured by detection of the oxidation of NADH at the electrode surface. A calibration curve for LDH in the 0.005-0.12 U range has been obtained with a repeatability of 3% and a reproducibility of 10%. The probe exhibited good selectivity (response of LD-2 was 18% of LD-1) which was further improved using thermal treatment of the membranes. Measurement of LD-1 content in human control serum with the developed procedure gave a LD-1 concentration in the reported assigned range.},
added-at = {2006-07-07T01:10:50.000+0200},
address = {Chemistry Department, University College Cork, Ireland.},
author = {Kelly, S. and Compagnone, D. and Guilbault, G.},
biburl = {https://www.bibsonomy.org/bibtex/2db2f6d5ec80243ade803b33acd2c4368/biblio24},
citeulike-article-id = {470524},
doi = {10.1016/S0956-5663(97)00105-X},
interhash = {dc421602c45b85eeaa85cfa0546fd542},
intrahash = {db2f6d5ec80243ade803b33acd2c4368},
issn = {0956-5663},
journal = {Biosens Bioelectron},
keywords = {amperometry lactate_dehydrogenase immunoassay immunosensor ag_detection electrochemistry immunoelectrode},
month = {February},
number = 2,
pages = {173--179},
priority = {2},
timestamp = {2006-07-07T01:10:50.000+0200},
title = {Amperometric immunosensor for lactate dehydrogenase LD-1.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9597734},
volume = 13,
year = 1998
}