1. Confocal Ca$^2+$ imaging was used to measure spontaneous release
events (Ca$^2+$ sparks) in fluo-3-loaded isolated rat ventricular
myocytes. 2. The microscopic Ca$^2+$ release flux underlying
Ca$^2+$ sparks was derived by adapting the methods used previously
to describe macroscopic Ca$^2+$ release from cell-averaged Ca$^2+$
transients. 3. The magnitude of the local release fluxes varied from
2 to 5 microM ms-1, depending on SR Ca$^2+$ loading conditions.
Following spontaneous activation, the release flux rapidly decayed
(tau = 6-12 ms). The rate of termination of release flux was found
to be directly related to the magnitude of the flux (r2 = 0.88).
4. The rate of termination of local release flux was slowed in the
presence of FK506, a compound that is known to reduce inactivation
of SR Ca$^2+$ channels in vitro. 5. These results suggest that
termination of release flux during sparks is not due to a spontaneous
stochastic decay process or local depletion of Ca$^2+$ from the
SR, but rather involves an active extinguishing mechanism such as
Ca$^2+$-dependent inactivation or adaptation.
%0 Journal Article
%1 Luky_1998_667
%A Lukyanenko, V.
%A Wiesner, T. F.
%A Gyorke, S.
%D 1998
%J J. Physiol.
%K 9508828 Algorithms, Aniline Animals, Calcium Calcium, Cells, Channels, Chemical, Compounds, Confocal, Cultured, Dyes, Fluorescent Gov't, Heart Heart, Kinetics, Microscopy, Models, Non-U.S. P.H.S., Rats, Reproducibility Research Results, Reticulum, Sarcoplasmic Sprague-Dawley, Support, Tacrolimus, U.S. Ventricles, Xanthenes, of
%P 667--677
%T Termination of Ca$^2+$ release during Ca$^2+$ sparks in rat
ventricular myocytes.
%U http://jp.physoc.org/cgi/content/full/507/3/667
%V 507 ( Pt 3)
%X 1. Confocal Ca$^2+$ imaging was used to measure spontaneous release
events (Ca$^2+$ sparks) in fluo-3-loaded isolated rat ventricular
myocytes. 2. The microscopic Ca$^2+$ release flux underlying
Ca$^2+$ sparks was derived by adapting the methods used previously
to describe macroscopic Ca$^2+$ release from cell-averaged Ca$^2+$
transients. 3. The magnitude of the local release fluxes varied from
2 to 5 microM ms-1, depending on SR Ca$^2+$ loading conditions.
Following spontaneous activation, the release flux rapidly decayed
(tau = 6-12 ms). The rate of termination of release flux was found
to be directly related to the magnitude of the flux (r2 = 0.88).
4. The rate of termination of local release flux was slowed in the
presence of FK506, a compound that is known to reduce inactivation
of SR Ca$^2+$ channels in vitro. 5. These results suggest that
termination of release flux during sparks is not due to a spontaneous
stochastic decay process or local depletion of Ca$^2+$ from the
SR, but rather involves an active extinguishing mechanism such as
Ca$^2+$-dependent inactivation or adaptation.
@article{Luky_1998_667,
abstract = {1. Confocal {C}a$^{2+}$ imaging was used to measure spontaneous release
events ({C}a$^{2+}$ sparks) in fluo-3-loaded isolated rat ventricular
myocytes. 2. The microscopic {C}a$^{2+}$ release flux underlying
{C}a$^{2+}$ sparks was derived by adapting the methods used previously
to describe macroscopic {C}a$^{2+}$ release from cell-averaged {C}a$^{2+}$
transients. 3. The magnitude of the local release fluxes varied from
2 to 5 microM ms-1, depending on SR {C}a$^{2+}$ loading conditions.
Following spontaneous activation, the release flux rapidly decayed
(tau = 6-12 ms). The rate of termination of release flux was found
to be directly related to the magnitude of the flux (r2 = 0.88).
4. The rate of termination of local release flux was slowed in the
presence of FK506, a compound that is known to reduce inactivation
of SR {C}a$^{2+}$ channels in vitro. 5. These results suggest that
termination of release flux during sparks is not due to a spontaneous
stochastic decay process or local depletion of {C}a$^{2+}$ from the
SR, but rather involves an active extinguishing mechanism such as
{C}a$^{2+}$-dependent inactivation or adaptation.},
added-at = {2009-06-03T11:20:58.000+0200},
author = {Lukyanenko, V. and Wiesner, T. F. and Gyorke, S.},
biburl = {https://www.bibsonomy.org/bibtex/2dba782ab2180c6cf6a58ad5204ad0ca5/hake},
description = {The whole bibliography file I use.},
file = {Luky_1998_667.pdf:Luky_1998_667.pdf:PDF},
interhash = {dd1d33270aded21b6c0427af1ece19ec},
intrahash = {dba782ab2180c6cf6a58ad5204ad0ca5},
journal = {J. Physiol.},
keywords = {9508828 Algorithms, Aniline Animals, Calcium Calcium, Cells, Channels, Chemical, Compounds, Confocal, Cultured, Dyes, Fluorescent Gov't, Heart Heart, Kinetics, Microscopy, Models, Non-U.S. P.H.S., Rats, Reproducibility Research Results, Reticulum, Sarcoplasmic Sprague-Dawley, Support, Tacrolimus, U.S. Ventricles, Xanthenes, of},
month = Mar,
pages = {667--677},
pmid = {9508828},
timestamp = {2009-06-03T11:21:21.000+0200},
title = {Termination of {C}a$^{2+}$ release during {C}a$^{2+}$ sparks in rat
ventricular myocytes.},
url = {http://jp.physoc.org/cgi/content/full/507/3/667},
volume = {507 ( Pt 3)},
year = 1998
}