%0 Journal Article %1 citeulike:797282 %A Rapicault, S. %A Limoges, B. %A Degrand, C. %C Laboratoire d'Electrochimie Organique, Université Blaise Pascal de Clermont-Ferrand, Aubière, France. %D 1996 %J Anal Chem %K labeled_mediator electrochemistry %N 5 %P 930--935 %T Renewable perfluorosulfonated ionomer carbon paste electrode for competitive homogeneous electrochemical immunoassays using a redox cationic labeled hapten. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8779445 %V 68 %X A Nafion-loaded carbon paste electrode (CPE) was prepared and its square wave voltammetric response at a fresh surface was optimized using (ferrocenylmethyl)-trimethylammonium iodide (1) as a model of a cation that accumulates irreversibly. Low current responses could be obtained reproducibly without preconditioning at a CPE loaded with 1-octanol and neutralized Nafion. Use of the former made the method more sensitive, and use of the latter gave lower background currents and stable analytical response over a long period of Nafion-loaded CPE use. Under optimized conditions and using a 5 min accumulation period, the modified electrode gave a linear current response over the range of 5 nM to 1.5 microM 1, a detection limit of 5 nM 1 and a relative standard deviation of 5% for 0.5 microM 1. Using these optimized conditions, the competitive homogeneous immunoassay of phenytoin as a model hapten was carried out with cobaltocenium-labeled phenytoin as tracer. Clinical serum samples (5 microL) of phenytoin at therapeutic concentrations (20-80 microM) could be analyzed using this method.

@article{citeulike:797282, abstract = {A Nafion-loaded carbon paste electrode (CPE) was prepared and its square wave voltammetric response at a fresh surface was optimized using (ferrocenylmethyl)-trimethylammonium iodide (1) as a model of a cation that accumulates irreversibly. Low current responses could be obtained reproducibly without preconditioning at a CPE loaded with 1-octanol and neutralized Nafion. Use of the former made the method more sensitive, and use of the latter gave lower background currents and stable analytical response over a long period of Nafion-loaded CPE use. Under optimized conditions and using a 5 min accumulation period, the modified electrode gave a linear current response over the range of 5 nM to 1.5 microM 1, a detection limit of 5 nM 1 and a relative standard deviation of 5% for 0.5 microM 1. Using these optimized conditions, the competitive homogeneous immunoassay of phenytoin as a model hapten was carried out with cobaltocenium-labeled phenytoin as tracer. Clinical serum samples (5 microL) of phenytoin at therapeutic concentrations (20-80 microM) could be analyzed using this method.}, added-at = {2006-08-15T13:00:25.000+0200}, address = {Laboratoire d'Electrochimie Organique, Université Blaise Pascal de Clermont-Ferrand, Aubière, France.}, author = {Rapicault, S. and Limoges, B. and Degrand, C.}, biburl = {https://www.bibsonomy.org/bibtex/2dd5bc7878ea9d5ec0fdce3f6d9a79947/biblio24}, citeulike-article-id = {797282}, interhash = {85a952e480b02d1a5ec6e26dd8c45b40}, intrahash = {dd5bc7878ea9d5ec0fdce3f6d9a79947}, issn = {0003-2700}, journal = {Anal Chem}, keywords = {labeled_mediator electrochemistry}, month = {March}, number = 5, pages = {930--935}, priority = {2}, timestamp = {2006-08-15T13:00:25.000+0200}, title = {Renewable perfluorosulfonated ionomer carbon paste electrode for competitive homogeneous electrochemical immunoassays using a redox cationic labeled hapten.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=8779445}, volume = 68, year = 1996 }