Quantification of the tissue levels and function of the G-protein
regulator phosducin-like protein (PhlP)
S. Schroder, und M. Lohse. Naunyn Schmiedebergs Arch Pharmacol, 362 (4-5):
435-9(November 2000)Schroder, S Lohse, M J Research Support, Non-U.S. Gov't Germany Naunyn-Schmiedeberg's
archives of pharmacology Naunyn Schmiedebergs Arch Pharmacol. 2000
Nov;362(4-5):435-9..
Zusammenfassung
Phosducin-like protein is a protein with wide-spread expression that
has been shown to be capable of inhibiting G-protein function in
vitro. However, it is not clear whether it is expressed in sufficient
amounts to actually exert such functions in vivo. Here we quantify
the expression of the short and the long splice variants of phosducin-like
protein, PhlPs and PhlP1. Western blots of various rat tissues showed
that PhlP1 was by far the dominant splice variant; its levels were
1.5-2 pmol/mg cytosolic protein in brain, liver and kidney, and about
0.5 pmol/mg cytosolic protein in lung, heart and skeletal muscle.
These values correspond to concentrations of 150-200 nM and 50 nM,
respectively. The levels of PhlPs were about 20-fold lower. Recombinant
phosducin, PhlP1 and PhlPs inhibited the interaction between G-protein
alpha- und betagamma-subunits with IC50-values of 6 nM, 6 nM and
90 nM, respectively, as determined by Gbetagamma-dependent ADP-ribosylation
of Galphai1 by pertussis-toxin. Thus, tissue concentrations of PhlP1
are clearly sufficient to affect G-protein function in vivo, while
the expression levels and the Gbetagamma-affinity of PhlPs are most
likely too low to have significant inhibitory effects on Gbetagamma
(G-protein betagamma-subunits).
Schroder, S Lohse, M J Research Support, Non-U.S. Gov't Germany Naunyn-Schmiedeberg's
archives of pharmacology Naunyn Schmiedebergs Arch Pharmacol. 2000
Nov;362(4-5):435-9.
%0 Journal Article
%1 Schroder2000
%A Schroder, S.
%A Lohse, M. J.
%D 2000
%J Naunyn Schmiedebergs Arch Pharmacol
%K Animals Carrier GTP-Binding Nerve Protein Proteins/*analysis/physiology Proteins/*physiology Rats Subunits Tissue
%N 4-5
%P 435-9
%T Quantification of the tissue levels and function of the G-protein
regulator phosducin-like protein (PhlP)
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11111839
%V 362
%X Phosducin-like protein is a protein with wide-spread expression that
has been shown to be capable of inhibiting G-protein function in
vitro. However, it is not clear whether it is expressed in sufficient
amounts to actually exert such functions in vivo. Here we quantify
the expression of the short and the long splice variants of phosducin-like
protein, PhlPs and PhlP1. Western blots of various rat tissues showed
that PhlP1 was by far the dominant splice variant; its levels were
1.5-2 pmol/mg cytosolic protein in brain, liver and kidney, and about
0.5 pmol/mg cytosolic protein in lung, heart and skeletal muscle.
These values correspond to concentrations of 150-200 nM and 50 nM,
respectively. The levels of PhlPs were about 20-fold lower. Recombinant
phosducin, PhlP1 and PhlPs inhibited the interaction between G-protein
alpha- und betagamma-subunits with IC50-values of 6 nM, 6 nM and
90 nM, respectively, as determined by Gbetagamma-dependent ADP-ribosylation
of Galphai1 by pertussis-toxin. Thus, tissue concentrations of PhlP1
are clearly sufficient to affect G-protein function in vivo, while
the expression levels and the Gbetagamma-affinity of PhlPs are most
likely too low to have significant inhibitory effects on Gbetagamma
(G-protein betagamma-subunits).
@article{Schroder2000,
abstract = {Phosducin-like protein is a protein with wide-spread expression that
has been shown to be capable of inhibiting G-protein function in
vitro. However, it is not clear whether it is expressed in sufficient
amounts to actually exert such functions in vivo. Here we quantify
the expression of the short and the long splice variants of phosducin-like
protein, PhlPs and PhlP1. Western blots of various rat tissues showed
that PhlP1 was by far the dominant splice variant; its levels were
1.5-2 pmol/mg cytosolic protein in brain, liver and kidney, and about
0.5 pmol/mg cytosolic protein in lung, heart and skeletal muscle.
These values correspond to concentrations of 150-200 nM and 50 nM,
respectively. The levels of PhlPs were about 20-fold lower. Recombinant
phosducin, PhlP1 and PhlPs inhibited the interaction between G-protein
alpha- und betagamma-subunits with IC50-values of 6 nM, 6 nM and
90 nM, respectively, as determined by Gbetagamma-dependent ADP-ribosylation
of Galphai1 by pertussis-toxin. Thus, tissue concentrations of PhlP1
are clearly sufficient to affect G-protein function in vivo, while
the expression levels and the Gbetagamma-affinity of PhlPs are most
likely too low to have significant inhibitory effects on Gbetagamma
(G-protein betagamma-subunits).},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Schroder, S. and Lohse, M. J.},
biburl = {https://www.bibsonomy.org/bibtex/2dd71f585d6d7006a173313434ac08642/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {68061c6430f1648b1d0c5a2d8a6df491},
intrahash = {dd71f585d6d7006a173313434ac08642},
issn = {0028-1298 (Print) 0028-1298 (Linking)},
journal = {Naunyn Schmiedebergs Arch Pharmacol},
keywords = {Animals Carrier GTP-Binding Nerve Protein Proteins/*analysis/physiology Proteins/*physiology Rats Subunits Tissue},
month = Nov,
note = {Schroder, S Lohse, M J Research Support, Non-U.S. Gov't Germany Naunyn-Schmiedeberg's
archives of pharmacology Naunyn Schmiedebergs Arch Pharmacol. 2000
Nov;362(4-5):435-9.},
number = {4-5},
pages = {435-9},
shorttitle = {Quantification of the tissue levels and function of the G-protein
regulator phosducin-like protein (PhlP)},
timestamp = {2010-12-14T18:12:31.000+0100},
title = {Quantification of the tissue levels and function of the G-protein
regulator phosducin-like protein (PhlP)},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11111839},
volume = 362,
year = 2000
}