%0 Journal Article %1 citeulike:470441 %A Kannuck, R. M. %A Bellama, J. M. %A Durst, R. A. %D 1988 %J Analytical Chemistry %K l-i-l-a complement immunoassay homogeneous liposome electrochemistry %N 2 %P 142--147 %R 10.1021/ac00153a009 %T Measurement of liposome-released ferrocyanide by a dual-function polymer modified electrode. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3348479 %V 60 %X Potasslun ferrocyanide is encapsulated in the aqueous cavity of spherical phospholipid bilayer vesicles (Ilposomes) at concentrations of approximateiy 10‘ moiecuies/liposome. Physlcai parameters and staMiity of these structures are detennined by electrochemical and spectroscopk methods. The eiectroactive marker ions (ferrocyanide) are released from within the liposome by either the additlon of surfactant or the complement lysis of the membrane. The classical compiement pathway is an antigenlantlbody-specific reaction that occurs when an antigen-sensltlzed llposome Immunospecifically binds with a correspondlng antibody in the presence of certain serum proteins (complement). The release of encapsulated ferrocyanide is monitored by differential pulse voitammetry. Prelknlnary investigations with an lon-exchange polymer modified electrode demonstrate the ability to preconcentrate the released marker at the electrode surface as well as the necessity of a polymer film to protect the surface from fouling during serum-mediated lysis.

@article{citeulike:470441, abstract = {Potasslun ferrocyanide is encapsulated in the aqueous cavity of spherical phospholipid bilayer vesicles (Ilposomes) at concentrations of approximateiy 10‘ moiecuies/liposome. Physlcai parameters and staMiity of these structures are detennined by electrochemical and spectroscopk methods. The eiectroactive marker ions (ferrocyanide) are released from within the liposome by either the additlon of surfactant or the complement lysis of the membrane. The classical compiement pathway is an antigenlantlbody-specific reaction that occurs when an antigen-sensltlzed llposome Immunospecifically binds with a correspondlng antibody in the presence of certain serum proteins (complement). The release of encapsulated ferrocyanide is monitored by differential pulse voitammetry. Prelknlnary investigations with an lon-exchange polymer modified electrode demonstrate the ability to preconcentrate the released marker at the electrode surface as well as the necessity of a polymer film to protect the surface from fouling during serum-mediated lysis.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Kannuck, R. M. and Bellama, J. M. and Durst, R. A.}, biburl = {https://www.bibsonomy.org/bibtex/2df0eb469f63c168a0d038e89068a98c3/biblio24}, citeulike-article-id = {470441}, doi = {10.1021/ac00153a009}, interhash = {b0cde477e8ce668d56045bf4abf7097a}, intrahash = {df0eb469f63c168a0d038e89068a98c3}, issn = {0003-2700}, journal = {Analytical Chemistry}, keywords = {l-i-l-a complement immunoassay homogeneous liposome electrochemistry}, month = {January}, number = 2, pages = {142--147}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Measurement of liposome-released ferrocyanide by a dual-function polymer modified electrode.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=3348479}, volume = 60, year = 1988 }