%0 Journal Article %1 Hu.200702 %A Hu, Ying %A Rahlfs, Stefan %A Mersch-Sundermann, Volker %A Becker, Katja %D 2007/02// %J Biol Chem %K Apoptosis Carcinoma Cell_Count Cell_Cycle Cell_Proliferation Cultured Dose-Response_Relationship Drug Genetic Glutathione Humans IFZ Lung_Neoplasms Messenger Non-Small-Cell_Lung Oxidation-Reduction Phosphotransferases RNA Reverse_Transcriptase_Polymerase_Chain_Reaction Sensitivity_and_Specificity Stilbenes Structure-Activity_Relationship Transcription Tumor_Cells glutathione_peroxidase %N 2 %P 207-219 %T Resveratrol modulates mRNA transcripts of genes related to redox metabolism and cell proliferation in non-small-cell lung carcinoma cells %V 388 %X Resveratrol is a polyphenolic chemopreventive agent that has been shown to influence cellular redox reactions. As a systematic approach to elucidating the complex effects of resveratrol on eukaryotic cells, we studied its dose-dependent effects on the transcript levels of genes and activities of enzymes related to redox metabolism, cell cycle regulation, and apoptotic cascades in the cancer cell line A549. Glutathione peroxidase (GPx)1 mRNA levels, as well as GPx and thioredoxin reductase (TrxR) activities, were significantly increased after resveratrol treatment, whereas total glutathione concentrations decreased. Increased transcript levels were also detected for selenophosphate synthetase 2 and superoxide dismutase 2. However, mRNA levels of thioredoxin, TrxR, glutathione reductase, glutathione S-transferase, superoxide dismutase 1, and catalase were not altered. Among the 12 genes studied that are related to the cell cycle, differentiation and apoptosis, mRNA levels of six genes, i

@article{Hu.200702, abstract = {Resveratrol is a polyphenolic chemopreventive agent that has been shown to influence cellular redox reactions. As a systematic approach to elucidating the complex effects of resveratrol on eukaryotic cells, we studied its dose-dependent effects on the transcript levels of genes and activities of enzymes related to redox metabolism, cell cycle regulation, and apoptotic cascades in the cancer cell line A549. Glutathione peroxidase (GPx)1 mRNA levels, as well as GPx and thioredoxin reductase (TrxR) activities, were significantly increased after resveratrol treatment, whereas total glutathione concentrations decreased. Increased transcript levels were also detected for selenophosphate synthetase 2 and superoxide dismutase 2. However, mRNA levels of thioredoxin, TrxR, glutathione reductase, glutathione S-transferase, superoxide dismutase 1, and catalase were not altered. Among the 12 genes studied that are related to the cell cycle, differentiation and apoptosis, mRNA levels of six genes, i}, added-at = {2008-10-14T16:07:18.000+0200}, author = {Hu, Ying and Rahlfs, Stefan and Mersch-Sundermann, Volker and Becker, Katja}, biburl = {https://www.bibsonomy.org/bibtex/2678af63d13353f09e47f49eeee29f469/nutribiochem}, interhash = {2e22181c6ee4abceafeea07147b1cd28}, intrahash = {678af63d13353f09e47f49eeee29f469}, journal = {Biol Chem}, keywords = {Apoptosis Carcinoma Cell_Count Cell_Cycle Cell_Proliferation Cultured Dose-Response_Relationship Drug Genetic Glutathione Humans IFZ Lung_Neoplasms Messenger Non-Small-Cell_Lung Oxidation-Reduction Phosphotransferases RNA Reverse_Transcriptase_Polymerase_Chain_Reaction Sensitivity_and_Specificity Stilbenes Structure-Activity_Relationship Transcription Tumor_Cells glutathione_peroxidase}, number = 2, pages = {207-219}, timestamp = {2008-10-14T16:08:42.000+0200}, title = {Resveratrol modulates mRNA transcripts of genes related to redox metabolism and cell proliferation in non-small-cell lung carcinoma cells}, volume = 388, year = {2007/02//} }