Oxidative biotransformations using oxygenases.
Curr Opin Chem Biol, 6 (2):
136--144 (April 2002)Abstract
Considerable progress has been made in manipulating oxidative biotransformations
using oxygenases. Substrate acceptance, catalytic activity, regioselectivity
and stereoselectivity have been improved significantly by substrate
engineering, enzyme engineering or biocatalyst screening. Preparative
biotransformations have been carried out to synthesize useful pharmaceutical
intermediates or chiral synthons on the gram to several-hundred-gram
scale, by use of whole cells of wild type or recombinant strains.
The synthetic application of oxygenases in vitro has been shown
to be possible by enzymatic or electrochemical regeneration of NADH
or NADPH.