A NADPH regenerating system using a new, protein engineered formate dehydrogenase (FDH) is investigated. The new enzyme is the first known NAD(P)H dependent FDH. It can be successfully employed in synthesis with other enzymes requiring a NADPH regeneration. All advantages of the known NAD(H) dependent FDH's in these enzyme coupled synthesis can now be transferred to NADP(H) dependent systems.
%0 Journal Article
%1 citeulike:867654
%A Seelbach, Karsten
%A Riebel, Bettina
%A Hummel, Werner
%A Kula, Maria-Regina
%A Tishkov, Vladimir I.
%A Egorov, Alexey M.
%A Wandrey, Christian
%A Kragl, Udo
%D 1996
%J Tetrahedron Letters
%K biocatalysis
%N 9
%P 1377--1380
%R 10.1016/0040-4039(96)00010-X
%T A novel, efficient regenerating method of NADPH using a new formate dehydrogenase
%U http://dx.doi.org/10.1016/0040-4039(96)00010-X
%V 37
%X A NADPH regenerating system using a new, protein engineered formate dehydrogenase (FDH) is investigated. The new enzyme is the first known NAD(P)H dependent FDH. It can be successfully employed in synthesis with other enzymes requiring a NADPH regeneration. All advantages of the known NAD(H) dependent FDH's in these enzyme coupled synthesis can now be transferred to NADP(H) dependent systems.
@article{citeulike:867654,
abstract = {A NADPH regenerating system using a new, protein engineered formate dehydrogenase (FDH) is investigated. The new enzyme is the first known NAD(P)H dependent FDH. It can be successfully employed in synthesis with other enzymes requiring a NADPH regeneration. All advantages of the known NAD(H) dependent FDH's in these enzyme coupled synthesis can now be transferred to NADP(H) dependent systems.},
added-at = {2006-10-23T11:08:30.000+0200},
author = {Seelbach, Karsten and Riebel, Bettina and Hummel, Werner and Kula, Maria-Regina and Tishkov, Vladimir I. and Egorov, Alexey M. and Wandrey, Christian and Kragl, Udo},
biburl = {https://www.bibsonomy.org/bibtex/2eabc2605fde609fdd4b194e15f0546f7/robert},
citeulike-article-id = {867654},
doi = {10.1016/0040-4039(96)00010-X},
interhash = {6c8eead13ab28d546e0781abfd0386d8},
intrahash = {eabc2605fde609fdd4b194e15f0546f7},
journal = {Tetrahedron Letters},
keywords = {biocatalysis},
month = {February},
number = 9,
pages = {1377--1380},
priority = {2},
timestamp = {2006-10-23T11:08:30.000+0200},
title = {A novel, efficient regenerating method of NADPH using a new formate dehydrogenase},
url = {http://dx.doi.org/10.1016/0040-4039(96)00010-X},
volume = 37,
year = 1996
}