%0 Journal Article %1 mokada-gopalIdentificationNovelSTAT6Regulated2017 %A Mokada-Gopal, Lavanya %A Boeser, Alexander %A Lehmann, Christian H. K. %A Drepper, Friedel %A Dudziak, Diana %A Warscheid, Bettina %A Voehringer, David %C United States %D 2017 %J Journal of immunology (Baltimore, Md. : 1950) %K *Proteome,*Transcriptome,Adaptor Antigen/genetics/metabolism,Mice,Mice Antigens/genetics/metabolism,Cell BALB C,Mice Cells/immunology,to_read Class Cultured,Gene Differentiation,Cells E/metabolism,Interleukin-4/*immunology,Ki-1 Expression Factor/genetics/immunology/*metabolism,Th2 Inbred Knockout,Phosphoproteins/genetics/metabolism,STAT6 Proteins Regulation/genetics/immunology,Immunoglobulin Signal Switching/genetics,Immunoglobulin Transcription Transducing/genetics/metabolism,Animals,B-Lymphocytes/*immunology,CD79 %N 9 %P 3737--3745 %R 10.4049/jimmunol.1601838 %T Identification of Novel STAT6-Regulated Proteins in Mouse B Cells by Comparative Transcriptome and Proteome Analysis. %V 198 %X The transcription factor STAT6 plays a key role in mediating signaling downstream of the receptors for IL-4 and IL-13. In B cells, STAT6 is required for class switch recombination to IgE and for germinal center formation during type 2 immune responses directed against allergens or helminths. In this study, we compared the transcriptomes and proteomes of primary mouse B cells from wild-type and STAT6-deficient mice cultured for 4 d in the presence or absence of IL-4. Microarray analysis revealed that 214 mRNAs were upregulated and 149 were downregulated $>$3-fold by IL-4 in a STAT6-dependent manner. Across all samples, $\sim$5000 proteins were identified by label-free quantitative liquid chromatography/mass spectrometry. A total of 149 proteins was found to be differentially expressed $>$3-fold between IL-4-stimulated wild-type and STAT6(-/-) B cells (75 upregulated and 74 downregulated). Comparative analysis of the proteome and transcriptome revealed that expression of these proteins was mainly regulated at the transcriptional level, which argues against a major role for posttranscriptional mechanisms that modulate the STAT6-dependent proteome. Nine proteins were selected for confirmation by flow cytometry or Western blot. We show that CD30, CD79b, SLP-76, DEC205, IL-5R$\alpha$, STAT5, and Thy1 are induced by IL-4 in a STAT6-dependent manner. In contrast, Syk and Fc receptor-like 1 were downregulated. This dataset provides a framework for further functional analysis of newly identified IL-4-regulated proteins in B cells that may contribute to germinal center formation and IgE switching in type 2 immunity.

@article{mokada-gopalIdentificationNovelSTAT6Regulated2017, abstract = {The transcription factor STAT6 plays a key role in mediating signaling downstream of the receptors for IL-4 and IL-13. In B cells, STAT6 is required for class switch recombination to IgE and for germinal center formation during type 2 immune responses directed against allergens or helminths. In this study, we compared the transcriptomes and proteomes of primary mouse B cells from wild-type and STAT6-deficient mice cultured for 4 d in the presence or absence of IL-4. Microarray analysis revealed that 214 mRNAs were upregulated and 149 were downregulated {$>$}3-fold by IL-4 in a STAT6-dependent manner. Across all samples, {$\sim$}5000 proteins were identified by label-free quantitative liquid chromatography/mass spectrometry. A total of 149 proteins was found to be differentially expressed {$>$}3-fold between IL-4-stimulated wild-type and STAT6(-/-) B cells (75 upregulated and 74 downregulated). Comparative analysis of the proteome and transcriptome revealed that expression of these proteins was mainly regulated at the transcriptional level, which argues against a major role for posttranscriptional mechanisms that modulate the STAT6-dependent proteome. Nine proteins were selected for confirmation by flow cytometry or Western blot. We show that CD30, CD79b, SLP-76, DEC205, IL-5R{$\alpha$}, STAT5, and Thy1 are induced by IL-4 in a STAT6-dependent manner. In contrast, Syk and Fc receptor-like 1 were downregulated. This dataset provides a framework for further functional analysis of newly identified IL-4-regulated proteins in B cells that may contribute to germinal center formation and IgE switching in type 2 immunity.}, added-at = {2024-05-17T13:01:35.000+0200}, address = {United States}, author = {{Mokada-Gopal}, Lavanya and Boeser, Alexander and Lehmann, Christian H. K. and Drepper, Friedel and Dudziak, Diana and Warscheid, Bettina and Voehringer, David}, biburl = {https://www.bibsonomy.org/bibtex/285b1a2edd869762c5b653f21532311e0/warscheidlab}, copyright = {Copyright {\copyright} 2017 by The American Association of Immunologists, Inc.}, doi = {10.4049/jimmunol.1601838}, interhash = {2eadb969986975fbd2fa8fd70d33d9de}, intrahash = {85b1a2edd869762c5b653f21532311e0}, issn = {1550-6606 0022-1767}, journal = {Journal of immunology (Baltimore, Md. : 1950)}, keywords = {*Proteome,*Transcriptome,Adaptor Antigen/genetics/metabolism,Mice,Mice Antigens/genetics/metabolism,Cell BALB C,Mice Cells/immunology,to_read Class Cultured,Gene Differentiation,Cells E/metabolism,Interleukin-4/*immunology,Ki-1 Expression Factor/genetics/immunology/*metabolism,Th2 Inbred Knockout,Phosphoproteins/genetics/metabolism,STAT6 Proteins Regulation/genetics/immunology,Immunoglobulin Signal Switching/genetics,Immunoglobulin Transcription Transducing/genetics/metabolism,Animals,B-Lymphocytes/*immunology,CD79}, langid = {english}, month = may, number = 9, pages = {3737--3745}, pmid = {28348271}, timestamp = {2024-05-17T13:01:35.000+0200}, title = {Identification of {{Novel STAT6-Regulated Proteins}} in {{Mouse B Cells}} by {{Comparative Transcriptome}} and {{Proteome Analysis}}.}, volume = 198, year = 2017 }