%0 Journal Article %1 citeulike:505008 %A Eugui, J. %A Logroño, M. J. %A Ruiz, R. %A Zugaza, C. %A Mirabel, J. L. %A Martínez, C. %C Laboratorio de Bioquímica, Hospital Txagorritxu, Vitoria, Spain. %D 1994 %J Clin Biochem %K immunoturbidimetry apob %N 4 %P 310--315 %R 10.1016/0009-9120(94)90035-3 %T Immunoturbidimetry of serum apolipoproteins A-I and B on the Cobas Bio centrifugal analyzer: method validation and reference values. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8001295 %V 27 %X We describe an immunoturbidimetric assay for estimating the concentration of apolipoproteins (apo) A-I and B in serum with a Cobas Bio centrifugal analyzer. Several analytical variables were studied: pH, temperature, wavelength, reaction time, sample pretreatment, antisera dilution, concentration of NaCl, phosphate buffer, and PEG 6000; a good optical response was obtained with small volumes of sample and antisera. Imprecision was low, with intrarun coefficient of variance (CV) from 1.1 to 2.8% and interrun CV from 1.8 to 3.2%. We demonstrated a good correlation between our method and radial immunodiffusion for apo A-I (r = 0.975) and apo B (r = 0.980). Reference values were calculated from a sample of 494 healthy subjects, and separate intervals for the ratio of apo A-I to apo B in gender and age subgroups were established.

@article{citeulike:505008, abstract = {We describe an immunoturbidimetric assay for estimating the concentration of apolipoproteins (apo) A-I and B in serum with a Cobas Bio centrifugal analyzer. Several analytical variables were studied: pH, temperature, wavelength, reaction time, sample pretreatment, antisera dilution, concentration of NaCl, phosphate buffer, and PEG 6000; a good optical response was obtained with small volumes of sample and antisera. Imprecision was low, with intrarun coefficient of variance (CV) from 1.1 to 2.8% and interrun CV from 1.8 to 3.2%. We demonstrated a good correlation between our method and radial immunodiffusion for apo A-I (r = 0.975) and apo B (r = 0.980). Reference values were calculated from a sample of 494 healthy subjects, and separate intervals for the ratio of apo A-I to apo B in gender and age subgroups were established.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Laboratorio de Bioquímica, Hospital Txagorritxu, Vitoria, Spain.}, author = {Eugui, J. and Logroño, M. J. and Ruiz, R. and Zugaza, C. and Mirabel, J. L. and Martínez, C.}, biburl = {https://www.bibsonomy.org/bibtex/2ebc0fddf0739c223ec65339692db95ce/biblio24}, citeulike-article-id = {505008}, doi = {10.1016/0009-9120(94)90035-3}, interhash = {915b5fe458d8a189937a2c3f76e4ff7c}, intrahash = {ebc0fddf0739c223ec65339692db95ce}, issn = {0009-9120}, journal = {Clin Biochem}, keywords = {immunoturbidimetry apob}, month = {August}, number = 4, pages = {310--315}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Immunoturbidimetry of serum apolipoproteins A-I and B on the Cobas Bio centrifugal analyzer: method validation and reference values.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=8001295}, volume = 27, year = 1994 }