%0 Journal Article %1 sharma2019antiinflammatory %A Sharma, Prerna %A Nanjaian, Mahadevan %A Kalra, Sanjeev %A Chidambaram, Kumarappan %D 2019 %E JPPRes, %J Journal of Pharmacy & Pharmacognosy Research %K acute-toxicity carrageenan chloroform-fraction free-radicals paw-edema β-sitosterol %N 6 %P 421-432 %T In vivo anti-inflammatory and in vitro antioxidant potential of leaf and bark fractions of Holoptelea integrifolia (Roxb.) Planch. %U http://jppres.com/jppres/pdf/vol7/jppres19.587_7.6.421.pdf %V 7 %X Context: Holoptelea integrifolia is most widely used as a traditional ethnomedicine in India by the local tribal, especially for the treatment of hyperglycemia and as an anti-inflammatory drug. Aims: To evaluate the acute oral toxicity, in vivo anti-inflammatory and in vitro antioxidant therapeutic potential of chloroform and n-butanol fractions of H. integrifolia barks and leaves using appropriate experimental models. Methods: Acute oral toxicity of chloroform and n-butanol fractions were evaluated by 423-acute toxic class method using OECD guidelines. The anti-inflammatory effect of chloroform and n-butanol fractions of H. integrifolia barks (CHIB) and leaves (CHIL) were evaluated at 400 mg/kg (po) on carrageenan-induced hind paw edema in Wistar rats. The observed anti-inflammatory effect was compared to the reference drug, indomethacin (10 mg/kg). The total reducing power of both fractions was determined in vitro and the results were compared with ascorbic acid. Results: The TLC analysis of the bark and leaf extracts revealed the presence of beta-sitosterol. The results of the evaluation of the anti-inflammatory activity of CHIB and CHIL fractions showed a time-dependent reduction of carrageenan-induced paw edema in statistical analysis. In addition, chloroform fraction of H. integrifolia was found to show notable antioxidant activity against hydrogen peroxide radicals scavenging. Conclusions: Experimentally studied CHIB and CHIL possessed interesting anti-inflammatory and antioxidant potential without inducing any apparent acute toxic properties. These pharmacological effects may be attributed, at least in part, to the presence of phenolic compounds/steroidal terpenoids. These results provide scientific support for the traditional use of H. integrifolia in the management of various inflammatory diseases.

@article{sharma2019antiinflammatory, abstract = {Context: Holoptelea integrifolia is most widely used as a traditional ethnomedicine in India by the local tribal, especially for the treatment of hyperglycemia and as an anti-inflammatory drug. Aims: To evaluate the acute oral toxicity, in vivo anti-inflammatory and in vitro antioxidant therapeutic potential of chloroform and n-butanol fractions of H. integrifolia barks and leaves using appropriate experimental models. Methods: Acute oral toxicity of chloroform and n-butanol fractions were evaluated by 423-acute toxic class method using OECD guidelines. The anti-inflammatory effect of chloroform and n-butanol fractions of H. integrifolia barks (CHIB) and leaves (CHIL) were evaluated at 400 mg/kg (po) on carrageenan-induced hind paw edema in Wistar rats. The observed anti-inflammatory effect was compared to the reference drug, indomethacin (10 mg/kg). The total reducing power of both fractions was determined in vitro and the results were compared with ascorbic acid. Results: The TLC analysis of the bark and leaf extracts revealed the presence of beta-sitosterol. The results of the evaluation of the anti-inflammatory activity of CHIB and CHIL fractions showed a time-dependent reduction of carrageenan-induced paw edema in statistical analysis. In addition, chloroform fraction of H. integrifolia was found to show notable antioxidant activity against hydrogen peroxide radicals scavenging. Conclusions: Experimentally studied CHIB and CHIL possessed interesting anti-inflammatory and antioxidant potential without inducing any apparent acute toxic properties. These pharmacological effects may be attributed, at least in part, to the presence of phenolic compounds/steroidal terpenoids. These results provide scientific support for the traditional use of H. integrifolia in the management of various inflammatory diseases.}, added-at = {2020-01-19T05:16:16.000+0100}, author = {Sharma, Prerna and Nanjaian, Mahadevan and Kalra, Sanjeev and Chidambaram, Kumarappan}, biburl = {https://www.bibsonomy.org/bibtex/2eefd51a62b61f6826fcfd4c637527171/jppres}, editor = {JPPRes}, interhash = {7121abd30642a9ab4e87de78fa7cdf07}, intrahash = {eefd51a62b61f6826fcfd4c637527171}, issn = {0719-4250}, journal = {Journal of Pharmacy & Pharmacognosy Research}, keywords = {acute-toxicity carrageenan chloroform-fraction free-radicals paw-edema β-sitosterol}, language = {Eng}, month = {Nov-Dec}, number = 6, pages = {421-432}, timestamp = {2020-01-19T05:16:16.000+0100}, title = {In vivo anti-inflammatory and in vitro antioxidant potential of leaf and bark fractions of Holoptelea integrifolia (Roxb.) Planch.}, url = {http://jppres.com/jppres/pdf/vol7/jppres19.587_7.6.421.pdf}, volume = 7, year = 2019 }