The non-haem chloroperoxidase gene (cpoF) from the pyrrolnitrin producer
Pseudomonas fluorescens BL914 was cloned using an oligonucleotide
derived from part of the N-terminal amino acid sequence of chloroperoxidase
(CPO-P) from Pseudomonas pyrrocina as a probe. Based on the overexpression
of cpoF in Escherichia coli and the stability of CPO-F against higher
temperatures and proteases, the enzyme was purified to homogeneity.
Partial characterization of the enzyme showed that it belongs to
the class of bacterial non-haem CPOs. To investigate the role of
CPO-F in pyrrolnitrin biosynthesis, the cpoF gene was inactivated
by insertion of a kanamycin cassette. Exchange of the chromosomal
cpoF gene against the disrupted copy had no influence on pyrrolnitrin
production demonstrating that CPO-F was not involved in pyrrolnitrin
biosynthesis.
%0 Journal Article
%1 citeulike:840781
%A Kirner, S.
%A Krauss, S.
%A Sury, G.
%A Lam, S. T.
%A Ligon, J. M.
%A van Pée, K. H.
%C Institut f��r Mikrobiologie, Universit��t Hohenheim, Stuttgart, Germany.
%D 1996
%J Microbiology
%K natural-products nitro-compounds biosynthesis
%P 2129--2135
%T The non-haem chloroperoxidase from Pseudomonas fluorescens
and its relationship to pyrrolnitrin biosynthesis.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8760926
%V 142 ( Pt 8)
%X The non-haem chloroperoxidase gene (cpoF) from the pyrrolnitrin producer
Pseudomonas fluorescens BL914 was cloned using an oligonucleotide
derived from part of the N-terminal amino acid sequence of chloroperoxidase
(CPO-P) from Pseudomonas pyrrocina as a probe. Based on the overexpression
of cpoF in Escherichia coli and the stability of CPO-F against higher
temperatures and proteases, the enzyme was purified to homogeneity.
Partial characterization of the enzyme showed that it belongs to
the class of bacterial non-haem CPOs. To investigate the role of
CPO-F in pyrrolnitrin biosynthesis, the cpoF gene was inactivated
by insertion of a kanamycin cassette. Exchange of the chromosomal
cpoF gene against the disrupted copy had no influence on pyrrolnitrin
production demonstrating that CPO-F was not involved in pyrrolnitrin
biosynthesis.
@article{citeulike:840781,
abstract = {The non-haem chloroperoxidase gene (cpoF) from the pyrrolnitrin producer
Pseudomonas fluorescens BL914 was cloned using an oligonucleotide
derived from part of the N-terminal amino acid sequence of chloroperoxidase
(CPO-P) from Pseudomonas pyrrocina as a probe. Based on the overexpression
of cpoF in Escherichia coli and the stability of CPO-F against higher
temperatures and proteases, the enzyme was purified to homogeneity.
Partial characterization of the enzyme showed that it belongs to
the class of bacterial non-haem CPOs. To investigate the role of
CPO-F in pyrrolnitrin biosynthesis, the cpoF gene was inactivated
by insertion of a kanamycin cassette. Exchange of the chromosomal
cpoF gene against the disrupted copy had no influence on pyrrolnitrin
production demonstrating that CPO-F was not involved in pyrrolnitrin
biosynthesis.},
added-at = {2007-02-02T11:54:15.000+0100},
address = {Institut f��r Mikrobiologie, Universit��t Hohenheim, Stuttgart, Germany.},
author = {Kirner, S. and Krauss, S. and Sury, G. and Lam, S. T. and Ligon, J. M. and van P{\'{e}}e, K. H.},
biburl = {https://www.bibsonomy.org/bibtex/2f7e86f6ac62febd28df83a283a8b5bc4/robert},
citeulike-article-id = {840781},
interhash = {7d7d1b3d7d8ee3eaa71cd843488905f9},
intrahash = {f7e86f6ac62febd28df83a283a8b5bc4},
issn = {1350-0872},
journal = {Microbiology},
keywords = {natural-products nitro-compounds biosynthesis},
month = {August},
pages = {2129--2135},
priority = {2},
timestamp = {2007-02-02T11:54:15.000+0100},
title = {The non-haem chloroperoxidase from \textit{Pseudomonas fluorescens}
and its relationship to pyrrolnitrin biosynthesis.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=8760926},
volume = {142 ( Pt 8)},
year = 1996
}