%0 Journal Article %1 Yu2008Highquality %A Yu, Haiyuan %A Braun, Pascal %A Yildirim, Muhammed A. %A Lemmens, Irma %A Venkatesan, Kavitha %A Sahalie, Julie %A Hirozane-Kishikawa, Tomoko %A Gebreab, Fana %A Li, Na %A Simonis, Nicolas %A Hao, Tong %A Rual, Jean-Francois F. %A Dricot, Amélie %A Vazquez, Alexei %A Murray, Ryan R. %A Simon, Christophe %A Tardivo, Leah %A Tam, Stanley %A Svrzikapa, Nenad %A Fan, Changyu %A de Smet, Anne-Sophie S. %A Motyl, Adriana %A Hudson, Michael E. %A Park, Juyong %A Xin, Xiaofeng %A Cusick, Michael E. %A Moore, Troy %A Boone, Charlie %A Snyder, Michael %A Roth, Frederick P. %A Barabási, Albert-László L. %A Tavernier, Jan %A Hill, David E. %A Vidal, Marc %D 2008 %I American Association for the Advancement of Science %J Science (New York, N.Y.) %K human interactome protein-protein-interactions %N 5898 %P 104--110 %R 10.1126/science.1158684 %T High-quality binary protein interaction map of the yeast interactome network. %U http://dx.doi.org/10.1126/science.1158684 %V 322 %X Current yeast interactome network maps contain several hundred molecular complexes with limited and somewhat controversial representation of direct binary interactions. We carried out a comparative quality assessment of current yeast interactome data sets, demonstrating that high-throughput yeast two-hybrid (Y2H) screening provides high-quality binary interaction information. Because a large fraction of the yeast binary interactome remains to be mapped, we developed an empirically controlled mapping framework to produce a "second-generation" high-quality, high-throughput Y2H data set covering approximately 20\% of all yeast binary interactions. Both Y2H and affinity purification followed by mass spectrometry (AP/MS) data are of equally high quality but of a fundamentally different and complementary nature, resulting in networks with different topological and biological properties. Compared to co-complex interactome models, this binary map is enriched for transient signaling interactions and intercomplex connections with a highly significant clustering between essential proteins. Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy.

@article{Yu2008Highquality, abstract = {Current yeast interactome network maps contain several hundred molecular complexes with limited and somewhat controversial representation of direct binary interactions. We carried out a comparative quality assessment of current yeast interactome data sets, demonstrating that high-throughput yeast two-hybrid ({Y2H}) screening provides high-quality binary interaction information. Because a large fraction of the yeast binary interactome remains to be mapped, we developed an empirically controlled mapping framework to produce a "second-generation" high-quality, high-throughput {Y2H} data set covering approximately 20\% of all yeast binary interactions. Both {Y2H} and affinity purification followed by mass spectrometry ({AP}/{MS}) data are of equally high quality but of a fundamentally different and complementary nature, resulting in networks with different topological and biological properties. Compared to co-complex interactome models, this binary map is enriched for transient signaling interactions and intercomplex connections with a highly significant clustering between essential proteins. Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Yu, Haiyuan and Braun, Pascal and Yildirim, Muhammed A. and Lemmens, Irma and Venkatesan, Kavitha and Sahalie, Julie and Hirozane-Kishikawa, Tomoko and Gebreab, Fana and Li, Na and Simonis, Nicolas and Hao, Tong and Rual, Jean-Fran\c{c}ois F. and Dricot, Am\'{e}lie and Vazquez, Alexei and Murray, Ryan R. and Simon, Christophe and Tardivo, Leah and Tam, Stanley and Svrzikapa, Nenad and Fan, Changyu and de Smet, Anne-Sophie S. and Motyl, Adriana and Hudson, Michael E. and Park, Juyong and Xin, Xiaofeng and Cusick, Michael E. and Moore, Troy and Boone, Charlie and Snyder, Michael and Roth, Frederick P. and Barab\'{a}si, Albert-L\'{a}szl\'{o} L. and Tavernier, Jan and Hill, David E. and Vidal, Marc}, biburl = {https://www.bibsonomy.org/bibtex/2d060e53ef28a8218228bb301a0d9d545/karthikraman}, citeulike-article-id = {3147104}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1158684}, citeulike-linkout-1 = {http://www.sciencemag.org/content/322/5898/104.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/322/5898/104.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/1158684//}, citeulike-linkout-4 = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2746753/}, citeulike-linkout-5 = {http://view.ncbi.nlm.nih.gov/pubmed/18719252}, citeulike-linkout-6 = {http://www.hubmed.org/display.cgi?uids=18719252}, day = 3, doi = {10.1126/science.1158684}, interhash = {310c5bec6704f027aff40489b3b550b6}, intrahash = {d060e53ef28a8218228bb301a0d9d545}, issn = {1095-9203}, journal = {Science (New York, N.Y.)}, keywords = {human interactome protein-protein-interactions}, month = oct, number = 5898, pages = {104--110}, pmcid = {PMC2746753}, pmid = {18719252}, posted-at = {2014-12-30 06:30:39}, priority = {2}, publisher = {American Association for the Advancement of Science}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {High-quality binary protein interaction map of the yeast interactome network.}, url = {http://dx.doi.org/10.1126/science.1158684}, volume = 322, year = 2008 }