Article,

Rubrobacter aplysinae sp. nov. isolated from the marine sponge Aplysina aerophoba

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IJSEM, 64 (3): 705-709 (2013)

Abstract

A Gram-positive, non-spore-forming bacterium RV113T was isolated from the marine sponge Aplysina aerophoba. The 16S rRNA gene sequence analysis and similarity studies showed that strain RV113T belongs to the genus Rubrobacter, and is most closely related to Rubrobacter bracariensis VF70612 S1T 96.9% and more distantly related < 93% to all other Rubrobacter species. The peptidoglycan diaminoacid was lysine. Strain RV113T exhibited a quinone system with the predominant compound menaquinone MK-8. The polar lipid profile of RV113T consisted of the major compounds phosphatidylglycerol and two unidentified phosphoglycolipids. The major fatty acid was anteiso C17:0ω9. These chemotaxonomic traits are in excellent agreement with those of other Rubrobacter species. The results of physiological and biochemical tests allowed a clear phenotypic differentiation of strain RV113T from all other Rubrobacter species. RV113T merits species status, and we propose the name Rubrobacter aplysinae sp. nov. with the type strain RV113T = DSM 27440T = CECT 8425T.

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