%0 Journal Article %1 citeulike:495865 %A Luca, Nicolae %D 1989 %J United States Patent %K patent apob %T Method for determination of lipid moiety and apolipoprotein expressed epitope immunoreactivity on intact lipoprotein %V 5187068 %X Disclosed are methods that permit the determination of both lipid moiety (LM) and apolipoprotein expressed epitope immunoreactivity (EEI) of intact isolated lipoprotein species. One format of the method isolates a lipoprotein species by means of a ligand binding to a solid phase, and quantitates thereafter the LM and/or the EEI. A complementary variant format provides for nonquantitative study of a constant number of lipoprotein particles exploring the sizes of the particles (LM) and scanning the apolipoprotein epitopes (EEI). Disclosed also is a method using lipid moiety dynamics (LMD) and expressed epitopes immunoreactivity dynamics (EEID) to explore the lipid loading and unloading of the lipoprotein species during a fast-feeding cycle. Both the LMD and EEID of lipoprotein particles may be performed as a panel, individual or in any combination. This can be used to detect abnormalities in lipid metabolism that precede the hyperlipidemias associated with cardiovascular disease.

@article{citeulike:495865, abstract = {Disclosed are methods that permit the determination of both lipid moiety (LM) and apolipoprotein expressed epitope immunoreactivity (EEI) of intact isolated lipoprotein species. One format of the method isolates a lipoprotein species by means of a ligand binding to a solid phase, and quantitates thereafter the LM and/or the EEI. A complementary variant format provides for nonquantitative study of a constant number of lipoprotein particles exploring the sizes of the particles (LM) and scanning the apolipoprotein epitopes (EEI). Disclosed also is a method using lipid moiety dynamics (LMD) and expressed epitopes immunoreactivity dynamics (EEID) to explore the lipid loading and unloading of the lipoprotein species during a fast-feeding cycle. Both the LMD and EEID of lipoprotein particles may be performed as a panel, individual or in any combination. This can be used to detect abnormalities in lipid metabolism that precede the hyperlipidemias associated with cardiovascular disease.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Luca, Nicolae}, biburl = {https://www.bibsonomy.org/bibtex/26f751483b8804fc64fa831a2c3402de1/biblio24}, citeulike-article-id = {495865}, interhash = {3cf78a54dbfdf87730da4b8d1ba2af0b}, intrahash = {6f751483b8804fc64fa831a2c3402de1}, journal = {United States Patent}, keywords = {patent apob}, month = {June}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Method for determination of lipid moiety and apolipoprotein expressed epitope immunoreactivity on intact lipoprotein}, volume = 5187068, year = 1989 }