%0 Journal Article %1 taha_interlaboratory_2005 %A Taha, Muhamed-Kheir %A Alonso, Jean-Michel %A Cafferkey, Mary %A Caugant, Dominique A %A Clarke, Stuart C %A Diggle, Mathew A %A Fox, Andrew %A Frosch, Matthias %A Gray, Stephen J %A Guiver, Malcolm %A Heuberger, Sigrid %A Kalmusova, Jitka %A Kesanopoulos, Konstantinos %A Klem, Anne-Marie %A Kriz, Paula %A Marsh, John %A MĂślling, Paula %A Murphy, Karen %A OlcĂŠn, Per %A Sanou, Oumar %A Tzanakaki, Georgina %A Vogel, Ulrich %D 2005 %J Journal of Clinical Microbiology %K Adolescent, Adult, Bacterial, Burkina Chain Child, European Faso, Female, Genotype, Humans, Infant, Infections, Laboratories, Male, Meningitis, Meningococcal Meningococcal, Neisseria Polymerase Preschool, Reaction, Sensitivity Specificity Union, and meningitidis, {DNA}, %N 1 %P 144--149 %R 10.1128/JCM.43.1.144-149.2005 %T Interlaboratory comparison of PCR-based identification and genogrouping of Neisseria meningitidis %U http://www.ncbi.nlm.nih.gov/pubmed/15634963 %V 43 %X Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7\%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7\%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.

@article{taha_interlaboratory_2005, abstract = {Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the {EU-MenNet} program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes {(crgA}, {ctrA}, {16S} {rRNA}, and {porA).} The {PCR-based} nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7\%, respectively. Most of the laboratories also performed genogrouping assays {(siaD} and {mynB/sacC).} The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7\%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this {PCR.} The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these {PCRs.}}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Taha, {Muhamed-Kheir} and Alonso, {Jean-Michel} and Cafferkey, Mary and Caugant, Dominique A and Clarke, Stuart C and Diggle, Mathew A and Fox, Andrew and Frosch, Matthias and Gray, Stephen J and Guiver, Malcolm and Heuberger, Sigrid and Kalmusova, Jitka and Kesanopoulos, Konstantinos and Klem, {Anne-Marie} and Kriz, Paula and Marsh, John and MĂślling, Paula and Murphy, Karen and OlcĂŠn, Per and Sanou, Oumar and Tzanakaki, Georgina and Vogel, Ulrich}, biburl = {https://www.bibsonomy.org/bibtex/294373fbcd064652a42587dcc3302cf03/ag_vogel}, doi = {10.1128/JCM.43.1.144-149.2005}, interhash = {3cf9d4ba0bd39e49f70c4558434a5402}, intrahash = {94373fbcd064652a42587dcc3302cf03}, issn = {0095-1137}, journal = {Journal of Clinical Microbiology}, keywords = {Adolescent, Adult, Bacterial, Burkina Chain Child, European Faso, Female, Genotype, Humans, Infant, Infections, Laboratories, Male, Meningitis, Meningococcal Meningococcal, Neisseria Polymerase Preschool, Reaction, Sensitivity Specificity Union, and meningitidis, {DNA},}, month = jan, note = {{PMID:} 15634963}, number = 1, pages = {144--149}, timestamp = {2011-06-24T11:21:48.000+0200}, title = {Interlaboratory comparison of {PCR-based} identification and genogrouping of Neisseria meningitidis}, url = {http://www.ncbi.nlm.nih.gov/pubmed/15634963}, volume = 43, year = 2005 }