%0 Journal Article %1 citeulike:488379 %A Suzawa, T. %A Ikariyama, Y. %A Aizawa, M. %C Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan. %D 1994 %J Anal Chem %K ab_detection amperometry labeled_enzyme immunoassay homogeneous immunosensor ecia electrochemistry gox ferrocene %N 22 %P 3889--3894 %T Multilabeling of ferrocenes to a glucose oxidase-digoxin conjugate for the development of a homogeneous electroenzymatic immunoassay. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7810897 %V 66 %X A new homogeneous electroenzymatic immunoassay was developed to determine antibody concentration using glucose oxidase and ferrocene as enzymatic and electrochemical amplifier, respectively. Digoxin (Dig)-conjugated glucose oxidase (GOx) was modified with ferrocene (Fec) to form Fec-GOx-Dig conjugate. After immunocomplex formation between the Fec-GOx-Dig conjugate and the anti-digoxin antibody, the complex underwent less electrochemical reaction due to the steric hindrance of the antibody. The ferrocene multilabeled conjugate was provided for the determination of anti-digoxin antibody. Since the strategy taken here is based on a combined effect of GOx and ferrocene, i.e., enzymatic amplification by GOx and electrochemical amplification by multilabeled ferrocenes, the antibody concentration was determined in the range from 1/50 to 1/500 dilution.

@article{citeulike:488379, abstract = {A new homogeneous electroenzymatic immunoassay was developed to determine antibody concentration using glucose oxidase and ferrocene as enzymatic and electrochemical amplifier, respectively. Digoxin (Dig)-conjugated glucose oxidase (GOx) was modified with ferrocene (Fec) to form Fec-GOx-Dig conjugate. After immunocomplex formation between the Fec-GOx-Dig conjugate and the anti-digoxin antibody, the complex underwent less electrochemical reaction due to the steric hindrance of the antibody. The ferrocene multilabeled conjugate was provided for the determination of anti-digoxin antibody. Since the strategy taken here is based on a combined effect of GOx and ferrocene, i.e., enzymatic amplification by GOx and electrochemical amplification by multilabeled ferrocenes, the antibody concentration was determined in the range from 1/50 to 1/500 dilution.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.}, author = {Suzawa, T. and Ikariyama, Y. and Aizawa, M.}, biburl = {https://www.bibsonomy.org/bibtex/2558850786f47348e4b8d8aa604ca5bcb/biblio24}, citeulike-article-id = {488379}, interhash = {3e86b6e0a2fd8a38d8d4713fd8d909d0}, intrahash = {558850786f47348e4b8d8aa604ca5bcb}, issn = {0003-2700}, journal = {Anal Chem}, keywords = {ab_detection amperometry labeled_enzyme immunoassay homogeneous immunosensor ecia electrochemistry gox ferrocene}, month = {November}, number = 22, pages = {3889--3894}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Multilabeling of ferrocenes to a glucose oxidase-digoxin conjugate for the development of a homogeneous electroenzymatic immunoassay.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=7810897}, volume = 66, year = 1994 }