%0 Journal Article %1 palmer2019streptococcus %A Palmer, Sara R. %A Ren, Zhi %A Hwang, Geelsu %A Liu, Yuan %A Combs, Ashton %A Söderström, Bill %A Vasquez, Patricia Lara %A Khosravi, Yalda %A Brady, L. Jeannine %A Koo, Hyun %A Stoodley, Paul %D 2019 %E Silhavy, Thomas J. %I American Society for Microbiology %J Journal of Bacteriology %K 92c05-biophysics 92c10-biomechanics 92c70-microbiology biofilm %N 1 %R 10.1128/jb.00396-18 %T Streptococcus mutans yidC and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties %U https://journals.asm.org/doi/10.1128/jb.00396-18 %V 201 %X Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases. The deletion of either paralog attenuates virulence in vivo, but the mechanisms of disruption remain unclear. Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm. Compared to the wild type, the ΔyidC2 mutant lacked staining with fluorescent vancomycin at the division septum, while the ΔyidC1 mutant resembled the wild type. Additionally, the deletion of either yidC1 or yidC2 resulted in less insoluble glucan synthesis but produced more soluble glucans, especially at early and mid-exponential-growth phases. Alteration of glucan synthesis by both mutants yielded biofilms with less dry weight and insoluble EPS. In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture. The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear. Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal. YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.

@article{palmer2019streptococcus, abstract = {Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases. The deletion of either paralog attenuates virulence in vivo, but the mechanisms of disruption remain unclear. Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm. Compared to the wild type, the ΔyidC2 mutant lacked staining with fluorescent vancomycin at the division septum, while the ΔyidC1 mutant resembled the wild type. Additionally, the deletion of either yidC1 or yidC2 resulted in less insoluble glucan synthesis but produced more soluble glucans, especially at early and mid-exponential-growth phases. Alteration of glucan synthesis by both mutants yielded biofilms with less dry weight and insoluble EPS. In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture. The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear. Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal. YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.}, added-at = {2023-10-04T04:15:32.000+0200}, author = {Palmer, Sara R. and Ren, Zhi and Hwang, Geelsu and Liu, Yuan and Combs, Ashton and Söderström, Bill and Vasquez, Patricia Lara and Khosravi, Yalda and Brady, L. Jeannine and Koo, Hyun and Stoodley, Paul}, biburl = {https://www.bibsonomy.org/bibtex/2d8b26450f62d2435796dd5c874cbb244/gdmcbain}, doi = {10.1128/jb.00396-18}, editor = {Silhavy, Thomas J.}, interhash = {44af438f653a9413570eecabee33a695}, intrahash = {d8b26450f62d2435796dd5c874cbb244}, journal = {Journal of Bacteriology}, keywords = {92c05-biophysics 92c10-biomechanics 92c70-microbiology biofilm}, month = jan, number = 1, publisher = {American Society for Microbiology}, timestamp = {2023-10-04T04:15:32.000+0200}, title = {Streptococcus mutans yidC and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties}, url = {https://journals.asm.org/doi/10.1128/jb.00396-18}, volume = 201, year = 2019 }