%0 Journal Article %1 andersen_neisseria_1993 %A Andersen, B M %A Steigerwalt, A G %A O'Connor, S P %A Hollis, D G %A Weyant, R S %A Weaver, R E %A Brenner, D J %D 1993 %J Journal of Clinical Microbiology %K Acid Animals, Bacterial, Base Bites Composition, Data, Dogs, Hybridization, Infection Infections, Molecular Neisseria, Neisseriaceae Nucleic Ribosomal, Sequence Sequence, Stings, Wound and {16S,} {DNA,} {RNA,} %N 9 %P 2456--2466 %T Neisseria weaveri sp. nov., formerly CDC group M-5, a gram-negative bacterium associated with dog bite wounds %U http://www.ncbi.nlm.nih.gov/pubmed/8408570 %V 31 %X CDC group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. DNA-DNA relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the Centers for Disease Control and Prevention. One typical M-5 strain, 8142, was further studied by 16S rRNA sequencing. DNA from 40 of 53 strains showed 82 to 100\% relatedness (hydroxyapatite method) to labeled DNA from strain 8142. The guanine-plus-cytosine (G + C) content in 8 of the 41 highly related M-5 strains was 50.5 to 52 mol\%. These 41 strains were oxidase and catalase positive, nonfermentative, nitrite positive, nitrate negative, weakly phenylalanine deaminase positive, aerobic, and alpha-hemolytic (sheep blood). DNA from the 13 remaining strains showed only 7 to 46\% DNA relatedness to strain 8142. These 13 non-M-5 strains differed from the M-5 strains in G + C content, growth characteristics, and biochemical profiles. DNA from M-5 strain 8142 was most closely related to DNA from groups EF-4b (47\%) and EF-4a (45\%). 16S rRNA sequence analysis placed M-5 strain 8142 in the Neisseriaceae cluster of the beta-3 subgroup of the class Proteobacteria. It was most homologous (98.4 to 98.8\%) to Neisseria animalis, Neisseria flavescens, Neisseria canis, and Neisseria elongata. All data are consistent with M-5 being a new species of Neisseria, for which we propose the name Neisseria weaveri.

@article{andersen_neisseria_1993, abstract = {{CDC} group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. {DNA-DNA} relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the Centers for Disease Control and Prevention. One typical M-5 strain, 8142, was further studied by {16S} {rRNA} sequencing. {DNA} from 40 of 53 strains showed 82 to 100\% relatedness (hydroxyapatite method) to labeled {DNA} from strain 8142. The guanine-plus-cytosine {(G} + C) content in 8 of the 41 highly related M-5 strains was 50.5 to 52 mol\%. These 41 strains were oxidase and catalase positive, nonfermentative, nitrite positive, nitrate negative, weakly phenylalanine deaminase positive, aerobic, and alpha-hemolytic (sheep blood). {DNA} from the 13 remaining strains showed only 7 to 46\% {DNA} relatedness to strain 8142. These 13 {non-M-5} strains differed from the M-5 strains in G + C content, growth characteristics, and biochemical profiles. {DNA} from M-5 strain 8142 was most closely related to {DNA} from groups {EF-4b} (47\%) and {EF-4a} (45\%). {16S} {rRNA} sequence analysis placed M-5 strain 8142 in the Neisseriaceae cluster of the beta-3 subgroup of the class Proteobacteria. It was most homologous (98.4 to 98.8\%) to Neisseria animalis, Neisseria flavescens, Neisseria canis, and Neisseria elongata. All data are consistent with M-5 being a new species of Neisseria, for which we propose the name Neisseria weaveri.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Andersen, B M and Steigerwalt, A G and {O'Connor}, S P and Hollis, D G and Weyant, R S and Weaver, R E and Brenner, D J}, biburl = {https://www.bibsonomy.org/bibtex/207ef11316db199e1d3a998fe2307c90f/jelias}, interhash = {45d66c9e51e889ee8721007aef8f77e3}, intrahash = {07ef11316db199e1d3a998fe2307c90f}, issn = {0095-1137}, journal = {Journal of Clinical Microbiology}, keywords = {Acid Animals, Bacterial, Base Bites Composition, Data, Dogs, Hybridization, Infection Infections, Molecular Neisseria, Neisseriaceae Nucleic Ribosomal, Sequence Sequence, Stings, Wound and {16S,} {DNA,} {RNA,}}, month = sep, note = {{PMID:} 8408570}, number = 9, pages = {2456--2466}, timestamp = {2011-03-11T10:05:37.000+0100}, title = {Neisseria weaveri sp. nov., formerly {CDC} group M-5, a gram-negative bacterium associated with dog bite wounds}, url = {http://www.ncbi.nlm.nih.gov/pubmed/8408570}, volume = 31, year = 1993 }