%0 Journal Article %1 RN980 %A Huang, S. G. %A Ding, M. Q. %A Roelfsema, M. R. G. %A Dreyer, I. %A Scherzer, S. %A Al-Rasheid, K. A. S. %A Gao, S. Q. %A Nagel, G. %A Hedrich, R. %A Konrad, K. R. %D 2021 %J Science Advances %K abscisic-acid activation myOwn %N 28 %R ARTN eabg4619 10.1126/sciadv.abg4619 %T Optogenetic control of the guard cell membrane potential and stomatal movement by the light-gated anion channel ACR1 %U /brokenurl#<Go to ISI>://WOS:000672817500009 %V 7 %X Guard cells control the aperture of plant stomata, which are crucial for global fluxes of CO2 and water. In turn, guard cell anion channels are seen as key players for stomatal closure, but is activation of these channels sufficient to limit plant water loss? To answer this open question, we used an optogenetic approach based on the light-gated anion channelrhodopsin 1 (GtACR1). In tobacco guard cells that express GtACR1, blue- and green-light pulses elicit Cl- and NO3- currents of -1 to -2 nA. The anion currents depolarize the plasma membrane by 60 to 80 mV, which causes opening of voltage-gated K+ channels and the extrusion of K+. As a result, continuous stimulation with green light leads to loss of guard cell turgor and closure of stomata at conditions that provoke stomatal opening in wild type. GtACR1 optogenetics thus provides unequivocal evidence that opening of anion channels is sufficient to close stomata.

@article{RN980, abstract = {Guard cells control the aperture of plant stomata, which are crucial for global fluxes of CO2 and water. In turn, guard cell anion channels are seen as key players for stomatal closure, but is activation of these channels sufficient to limit plant water loss? To answer this open question, we used an optogenetic approach based on the light-gated anion channelrhodopsin 1 (GtACR1). In tobacco guard cells that express GtACR1, blue- and green-light pulses elicit Cl- and NO3- currents of -1 to -2 nA. The anion currents depolarize the plasma membrane by 60 to 80 mV, which causes opening of voltage-gated K+ channels and the extrusion of K+. As a result, continuous stimulation with green light leads to loss of guard cell turgor and closure of stomata at conditions that provoke stomatal opening in wild type. GtACR1 optogenetics thus provides unequivocal evidence that opening of anion channels is sufficient to close stomata.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Huang, S. G. and Ding, M. Q. and Roelfsema, M. R. G. and Dreyer, I. and Scherzer, S. and Al-Rasheid, K. A. S. and Gao, S. Q. and Nagel, G. and Hedrich, R. and Konrad, K. R.}, biburl = {https://www.bibsonomy.org/bibtex/2384f4af88d7c742dbbc8b1b7b4fa67be/rainerhedrich_2}, doi = {ARTN eabg4619 10.1126/sciadv.abg4619}, interhash = {4952230b1b3b7eb412938571b09917f2}, intrahash = {384f4af88d7c742dbbc8b1b7b4fa67be}, issn = {2375-2548}, journal = {Science Advances}, keywords = {abscisic-acid activation myOwn}, note = {Ti5bx Times Cited:21 Cited References Count:76}, number = 28, timestamp = {2024-02-14T14:38:32.000+0100}, title = {Optogenetic control of the guard cell membrane potential and stomatal movement by the light-gated anion channel ACR1}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000672817500009}, volume = 7, year = 2021 }