%0 Journal Article %1 Simiantonaki.2008 %A Simiantonaki, N. %A Jayasinghe, C. %A Michel-Schmidt, R. %A Peters, K. %A Hermanns, M. I. %A Kirkpatrick, C. J. %D 2008 %J Int.J.Oncol. %K 1 Adenocarcinoma Autocrine Breast C Carcinoma Cell Colorectal Communication Endothelial Expression Factor Gene Growth Human Humans Hypoxia Hypoxia-Inducible Line Lung Neoplasms Neoplastic Oxygen Receptor-1 Receptor-2 Receptor-3 Regulation Subunit Survival Tumor Up-Regulation Vascular alpha cells genetics metabolism pathology physiology protein %N 3 %P 585-592 %T Hypoxia-induced epithelial VEGF-C/VEGFR-3 upregulation in carcinoma cell lines %U PM:18292935 %V 32 %X Adaptation to hypoxia, a universal hallmark of carcinomas, is a critical step for tumor cell survival and growth. One of the principal regulators of hypoxia-responsive pathways is the transcription factor hypoxia-inducible factor-1 alpha (HIF-1 alpha). Currently, it is known that tumoral production of members of the vascular endothelial growth factor (VEGF)-family (VEGFs) may promote tumor growth and progression by acting on carcinoma cells that express the cognate receptors (VEGFRs). However, the influence of hypoxia in the formation of such a tumoral VEGF/VEGFR loop is not completely understood. In the present study we examined the potential existence of a HIF-1 alpha/VEGF/VEGFR autocrine loop on commonly occurring carcinomas. The experiments were performed on five colorectal carcinoma cell lines, one breast (MCF7) and one lung (A549) adenocarcinoma cell line under normoxic and oxygen stress conditions using HIF-1 alpha-EIA, VEGFs-ELISA as well as RT-PCR and immunofluorescence for VE

@article{Simiantonaki.2008, abstract = {Adaptation to hypoxia, a universal hallmark of carcinomas, is a critical step for tumor cell survival and growth. One of the principal regulators of hypoxia-responsive pathways is the transcription factor hypoxia-inducible factor-1 alpha (HIF-1 alpha). Currently, it is known that tumoral production of members of the vascular endothelial growth factor (VEGF)-family (VEGFs) may promote tumor growth and progression by acting on carcinoma cells that express the cognate receptors (VEGFRs). However, the influence of hypoxia in the formation of such a tumoral VEGF/VEGFR loop is not completely understood. In the present study we examined the potential existence of a HIF-1 alpha/VEGF/VEGFR autocrine loop on commonly occurring carcinomas. The experiments were performed on five colorectal carcinoma cell lines, one breast (MCF7) and one lung (A549) adenocarcinoma cell line under normoxic and oxygen stress conditions using HIF-1 alpha-EIA, VEGFs-ELISA as well as RT-PCR and immunofluorescence for VE}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Simiantonaki, N. and Jayasinghe, C. and Michel-Schmidt, R. and Peters, K. and Hermanns, M. I. and Kirkpatrick, C. J.}, biburl = {https://www.bibsonomy.org/bibtex/294c1263be4532fda2056dce70ae83ab4/kanefendt}, interhash = {4dc9f3c1402a31fa9b63d1d46271568f}, intrahash = {94c1263be4532fda2056dce70ae83ab4}, journal = {Int.J.Oncol.}, keywords = {1 Adenocarcinoma Autocrine Breast C Carcinoma Cell Colorectal Communication Endothelial Expression Factor Gene Growth Human Humans Hypoxia Hypoxia-Inducible Line Lung Neoplasms Neoplastic Oxygen Receptor-1 Receptor-2 Receptor-3 Regulation Subunit Survival Tumor Up-Regulation Vascular alpha cells genetics metabolism pathology physiology protein}, number = 3, pages = {585-592}, timestamp = {2010-02-05T11:28:50.000+0100}, title = {Hypoxia-induced epithelial VEGF-C/VEGFR-3 upregulation in carcinoma cell lines}, url = {PM:18292935}, volume = 32, year = 2008 }