%0 Journal Article %1 serruto_neisseria_2010 %A Serruto, Davide %A Spadafina, Tiziana %A Ciucchi, Laura %A Lewis, Lisa A %A Ram, Sanjay %A Tontini, Marta %A Santini, Laura %A Biolchi, Alessia %A Seib, Kate L %A Giuliani, Marzia M %A Donnelly, John J %A Berti, Francesco %A Savino, Silvana %A Scarselli, Maria %A Costantino, Paolo %A Kroll, J Simon %A O'Dwyer, Clíona %A Qiu, Jiazhou %A Plaut, Andrew G %A Moxon, Richard %A Rappuoli, Rino %A Pizza, Mariagrazia %A Aricò, Beatrice %D 2010 %J Proceedings of the National Academy of Sciences of the United States of America %K Acid Amino Antibodies, Antigens, Antimicrobial Bacterial, Blood Carrier Cationic Factors Humans, Infections, Lactoferrin, Meningococcal Neisseria Peptides, Proteins, Sequence, Vaccines, Virulence meningitidis, %N 8 %P 3770--3775 %R 10.1073/pnas.0915162107 %T Neisseria meningitidis GNA2132, a heparin-binding protein that induces protective immunity in humans %U http://www.ncbi.nlm.nih.gov/pubmed/20133713 %V 107 %X GNA2132 is a Neisseria meningitidis antigen of unknown function, discovered by reverse vaccinology, which has been shown to induce bactericidal antibodies in animal models. Here we show that this antigen induces protective immunity in humans and it is recognized by sera of patients after meningococcal disease. The protein binds heparin in vitro through an Arg-rich region and this property correlates with increased survival of the unencapsulated bacterium in human serum. Furthermore, two proteases, the meningococcal NalP and human lactoferrin, cleave the protein upstream and downstream from the Arg-rich region, respectively. We conclude that GNA2132 is an important protective antigen of N. meningitidis and we propose to rename it, Neisserial Heparin Binding Antigen (NHBA).

@article{serruto_neisseria_2010, abstract = {{GNA2132} is a Neisseria meningitidis antigen of unknown function, discovered by reverse vaccinology, which has been shown to induce bactericidal antibodies in animal models. Here we show that this antigen induces protective immunity in humans and it is recognized by sera of patients after meningococcal disease. The protein binds heparin in vitro through an Arg-rich region and this property correlates with increased survival of the unencapsulated bacterium in human serum. Furthermore, two proteases, the meningococcal {NalP} and human lactoferrin, cleave the protein upstream and downstream from the Arg-rich region, respectively. We conclude that {GNA2132} is an important protective antigen of N. meningitidis and we propose to rename it, Neisserial Heparin Binding Antigen {(NHBA).}}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Serruto, Davide and Spadafina, Tiziana and Ciucchi, Laura and Lewis, Lisa A and Ram, Sanjay and Tontini, Marta and Santini, Laura and Biolchi, Alessia and Seib, Kate L and Giuliani, Marzia M and Donnelly, John J and Berti, Francesco and Savino, Silvana and Scarselli, Maria and Costantino, Paolo and Kroll, J Simon and {O'Dwyer}, Clíona and Qiu, Jiazhou and Plaut, Andrew G and Moxon, Richard and Rappuoli, Rino and Pizza, Mariagrazia and Aricò, Beatrice}, biburl = {https://www.bibsonomy.org/bibtex/267438da4e1293be27a7e02f1e0d834e3/jelias}, doi = {10.1073/pnas.0915162107}, interhash = {595e968ef0c6bf3d9c7eef9e7ce5da30}, intrahash = {67438da4e1293be27a7e02f1e0d834e3}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {Acid Amino Antibodies, Antigens, Antimicrobial Bacterial, Blood Carrier Cationic Factors Humans, Infections, Lactoferrin, Meningococcal Neisseria Peptides, Proteins, Sequence, Vaccines, Virulence meningitidis,}, month = feb, note = {{PMID:} 20133713}, number = 8, pages = {3770--3775}, timestamp = {2011-03-11T10:06:41.000+0100}, title = {Neisseria meningitidis {GNA2132,} a heparin-binding protein that induces protective immunity in humans}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20133713}, volume = 107, year = 2010 }