%0 Journal Article %1 Mohle.1997 %A Mohle, R. %A Green, D. %A Moore, M. A. %A Nachman, R. L. %A Rafii, S. %D 1997 %J Proc.Natl.Acad.Sci.U.S.A %K A Alternative Blood Cell Cells Complex Cultured Cytokines Endothelial Endothelium Expression Factor Factors GPIIb-IIIa Gene Glycoprotein Growth Hematopoiesis Human Humans Immunohistochemistry Kinase Kinases Laboratories Line Lymphokines Megakaryocytes Neovascularization Physiologic Platelet Platelets Protein-Tyrosine Receptor Receptors Research Splicing Thrombin Vascular analysis cells cytology genetics metabolism pharmacology protein secretion %N 2 %P 663-668 %T Constitutive production and thrombin-induced release of vascular endothelial growth factor by human megakaryocytes and platelets %U PM:9012841 %V 94 %X We have shown that coculture of bone marrow microvascular endothelial cells with hematopoietic progenitor cells results in proliferation and differentiation of megakaryocytes. In these long-term cultures, bone marrow microvascular endothelial cell monolayers maintain their cellular integrity in the absence of exogenous endothelial growth factors. Because this interaction may involve paracrine secretion of cytokines, we evaluated megakaryocytic cells for secretion of cytokines, we evaluated megakaryocytic cells for secretion of vascular endothelial growth factor (VEGF). Megakaryocytes (CD41a+) were generated by ex vivo expansion of hematopoietic progenitor cells with kit-ligand and thrombopoietin for 10 days and further purified with immunomagnetic microbeads. Using reverse transcription-PCR, we showed that megakaryocytic cell lines (Dami, HEL) and purified megakaryocytes expressed mRNA of the three VEGF isoforms (121, 165, and 189 amino acids). Large quantities of VEGF (> 1 ng/10(6) ce

@article{Mohle.1997, abstract = {We have shown that coculture of bone marrow microvascular endothelial cells with hematopoietic progenitor cells results in proliferation and differentiation of megakaryocytes. In these long-term cultures, bone marrow microvascular endothelial cell monolayers maintain their cellular integrity in the absence of exogenous endothelial growth factors. Because this interaction may involve paracrine secretion of cytokines, we evaluated megakaryocytic cells for secretion of cytokines, we evaluated megakaryocytic cells for secretion of vascular endothelial growth factor (VEGF). Megakaryocytes (CD41a+) were generated by ex vivo expansion of hematopoietic progenitor cells with kit-ligand and thrombopoietin for 10 days and further purified with immunomagnetic microbeads. Using reverse transcription-PCR, we showed that megakaryocytic cell lines (Dami, HEL) and purified megakaryocytes expressed mRNA of the three VEGF isoforms (121, 165, and 189 amino acids). Large quantities of VEGF (> 1 ng/10(6) ce}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Mohle, R. and Green, D. and Moore, M. A. and Nachman, R. L. and Rafii, S.}, biburl = {https://www.bibsonomy.org/bibtex/2f877fdd017e4a982a2b7aa3216a89ebb/kanefendt}, interhash = {5ba37e771803c9eb0ed178e9c2798cf8}, intrahash = {f877fdd017e4a982a2b7aa3216a89ebb}, journal = {Proc.Natl.Acad.Sci.U.S.A}, keywords = {A Alternative Blood Cell Cells Complex Cultured Cytokines Endothelial Endothelium Expression Factor Factors GPIIb-IIIa Gene Glycoprotein Growth Hematopoiesis Human Humans Immunohistochemistry Kinase Kinases Laboratories Line Lymphokines Megakaryocytes Neovascularization Physiologic Platelet Platelets Protein-Tyrosine Receptor Receptors Research Splicing Thrombin Vascular analysis cells cytology genetics metabolism pharmacology protein secretion}, number = 2, pages = {663-668}, timestamp = {2010-02-05T11:28:46.000+0100}, title = {Constitutive production and thrombin-induced release of vascular endothelial growth factor by human megakaryocytes and platelets}, url = {PM:9012841}, volume = 94, year = 1997 }