%0 Journal Article %1 komatsu2011virus %A Komatsu, Teruyuki %A Qu, Xue %A Ihara, Hiromi %A Fujihara, Mitsuhiro %A Azuma, Hiroshi %A Ikeda, Hisami %D 2011 %J Journal of the American Chemical Society %K albumin lbl nanotubes virus %N 10 %P 3246-3248 %R 10.1021/ja1096122 %T Virus Trap in Human Serum Albumin Nanotube %U http://pubs.acs.org/doi/abs/10.1021/ja1096122 %V 133 %X Infectious hepatitis B virus (HBV), namely Dane particles (DPs), consists of a core nucleocapsid including genome DNA covered with an envelope of hepatitis B surface antigen (HBsAg). We report the synthesis, structure, and HBV-trapping capability of multilayered protein nanotubes having an anti-HBsAg antibody (HBsAb) layer as an internal wall. The nanotubes were prepared using an alternating layer-by-layer assembly of human serum albumin (HSA) and oppositely charged poly-l-arginine (PLA) into a nanoporous polycarbonate (PC) membrane (pore size, 400 nm), followed by depositions of poly-l-glutamic acid (PLG) and HBsAb. Subsequent dissolution of the PC template yielded (PLA/HSA)2PLA/PLG/HBsAb nanotubes (AbNTs). The SEM measurements revealed the formation of uniform hollow cylinders with a 414 ± 16 nm outer diameter and 59 ± 4 nm wall thickness. In an aqueous medium, the swelled nanotubes captured noninfectious spherical small particles of HBsAg (SPs); the binding constant was 3.5 × 107 M−1. Surprisingly, the amount of genome DNA in the HBV solution (HBsAg-positive plasma or DP-rich solution) decreased dramatically after incubation with the AbNTs (−3.9 log order), which implies that the infectious DPs were completely entrapped into the one-dimensional pore space of the AbNTs.

@article{komatsu2011virus, abstract = { Infectious hepatitis B virus (HBV), namely Dane particles (DPs), consists of a core nucleocapsid including genome DNA covered with an envelope of hepatitis B surface antigen (HBsAg). We report the synthesis, structure, and HBV-trapping capability of multilayered protein nanotubes having an anti-HBsAg antibody (HBsAb) layer as an internal wall. The nanotubes were prepared using an alternating layer-by-layer assembly of human serum albumin (HSA) and oppositely charged poly-l-arginine (PLA) into a nanoporous polycarbonate (PC) membrane (pore size, 400 nm), followed by depositions of poly-l-glutamic acid (PLG) and HBsAb. Subsequent dissolution of the PC template yielded (PLA/HSA)2PLA/PLG/HBsAb nanotubes (AbNTs). The SEM measurements revealed the formation of uniform hollow cylinders with a 414 ± 16 nm outer diameter and 59 ± 4 nm wall thickness. In an aqueous medium, the swelled nanotubes captured noninfectious spherical small particles of HBsAg (SPs); the binding constant was 3.5 × 107 M−1. Surprisingly, the amount of genome DNA in the HBV solution (HBsAg-positive plasma or DP-rich solution) decreased dramatically after incubation with the AbNTs (−3.9 log order), which implies that the infectious DPs were completely entrapped into the one-dimensional pore space of the AbNTs. }, added-at = {2011-05-31T08:24:14.000+0200}, author = {Komatsu, Teruyuki and Qu, Xue and Ihara, Hiromi and Fujihara, Mitsuhiro and Azuma, Hiroshi and Ikeda, Hisami}, biburl = {https://www.bibsonomy.org/bibtex/2acb2c6b44931cb5ae7818f466969bf87/saghi}, description = {Virus Trap in Human Serum Albumin Nanotube - Journal of the American Chemical Society (ACS Publications)}, doi = {10.1021/ja1096122}, eprint = {http://pubs.acs.org/doi/pdf/10.1021/ja1096122}, interhash = {5d0c717912875fbfc5f0f947f093e1a6}, intrahash = {acb2c6b44931cb5ae7818f466969bf87}, journal = {Journal of the American Chemical Society}, keywords = {albumin lbl nanotubes virus}, number = 10, pages = {3246-3248}, timestamp = {2011-05-31T08:24:14.000+0200}, title = {Virus Trap in Human Serum Albumin Nanotube}, url = {http://pubs.acs.org/doi/abs/10.1021/ja1096122}, volume = 133, year = 2011 }