%0 Journal Article %1 Hartl2006 %A Hartl, Dominik %A Griese, Matthias %A Kappler, Matthias %A Zissel, Gernot %A Reinhardt, Dietrich %A Rebhan, Christian %A Schendel, Dolores J. %A Krauss-Etschmann, Susanne %D 2006 %J J Allergy Clin Immunol %K pseudomonas mucosal cysticfibrosis pneumonia %N 1 %P 204--211 %R 10.1016/j.jaci.2005.09.023 %T Pulmonary T(H)2 response in Pseudomonas aeruginosa-infected patients with cystic fibrosis. %U http://dx.doi.org/10.1016/j.jaci.2005.09.023 %V 117 %X Pseudomonas aeruginosa infection determines the course of cystic fibrosis (CF) lung disease. Studies in human peripheral blood indicate that P aeruginosa infection is associated with a predominant T(H)2 immune response, whereas T(H)1 responses are accompanied by a better pulmonary outcome.Analyses of peripheral blood may not correspond directly with the local pulmonary immune response. Therefore, we asked whether the T(H)1/T(H)2 response is altered in bronchoalveolar lavage fluid from P aeruginosa-infected patients with CF.Bronchoalveolar lavage fluid was obtained from 12 patients with CF chronically infected with P aeruginosa, 11 noninfected patients with CF, and 8 healthy controls. Pulmonary CXCR3(+) (T(H)1) and CCR4(+) (T(H)2) expressing CD4(+) and CD8(+) lymphocytes were quantified by flow cytometry. Levels of T(H)1-associated (IL-2, IFN-gamma, IFN-gamma inducible T cell-alpha chemoattractant, Monokine induced by IFN-gamma, IFN-gamma inducible protein of 10 kd) and T(H)2-associated (IL-4, IL-5, IL-10, thymus and activation-regulated chemokine TARC, macrophage-derived chemokine) cytokines and chemokines and a panel of proinflammatory molecules were quantified at the protein level. Chemokines mRNA levels were assessed by real time RT-PCR.P aeruginosa-infected patients with CF had significantly higher levels of pulmonary CCR4(+)CD4(+) (T(H)2) cells, IL-4, IL-13, and TARC and lower levels of IFN-gamma compared with noninfected patients with CF and healthy controls. Bronchoalveolar lavage fluid levels of IL-4, IL-13, and TARC correlated inversely with FEV(1) in P aeruginosa-infected patients with CF.These results reveal the prevalence of a pulmonary T(H)2 immune response in P aeruginosa-infected patients with CF. The modulation of the pulmonary T(H)2 response in P aeruginosa infection may be an option for the treatment of P aeruginosa lung disease in patients with CF.

@article{Hartl2006, abstract = {Pseudomonas aeruginosa infection determines the course of cystic fibrosis (CF) lung disease. Studies in human peripheral blood indicate that P aeruginosa infection is associated with a predominant T(H)2 immune response, whereas T(H)1 responses are accompanied by a better pulmonary outcome.Analyses of peripheral blood may not correspond directly with the local pulmonary immune response. Therefore, we asked whether the T(H)1/T(H)2 response is altered in bronchoalveolar lavage fluid from P aeruginosa-infected patients with CF.Bronchoalveolar lavage fluid was obtained from 12 patients with CF chronically infected with P aeruginosa, 11 noninfected patients with CF, and 8 healthy controls. Pulmonary CXCR3(+) (T(H)1) and CCR4(+) (T(H)2) expressing CD4(+) and CD8(+) lymphocytes were quantified by flow cytometry. Levels of T(H)1-associated (IL-2, IFN-gamma, IFN-gamma inducible T cell-alpha chemoattractant, Monokine induced by IFN-gamma, IFN-gamma inducible protein of 10 kd) and T(H)2-associated (IL-4, IL-5, IL-10, thymus and activation-regulated chemokine [TARC], macrophage-derived chemokine) cytokines and chemokines and a panel of proinflammatory molecules were quantified at the protein level. Chemokines mRNA levels were assessed by real time RT-PCR.P aeruginosa-infected patients with CF had significantly higher levels of pulmonary CCR4(+)CD4(+) (T(H)2) cells, IL-4, IL-13, and TARC and lower levels of IFN-gamma compared with noninfected patients with CF and healthy controls. Bronchoalveolar lavage fluid levels of IL-4, IL-13, and TARC correlated inversely with FEV(1) in P aeruginosa-infected patients with CF.These results reveal the prevalence of a pulmonary T(H)2 immune response in P aeruginosa-infected patients with CF. The modulation of the pulmonary T(H)2 response in P aeruginosa infection may be an option for the treatment of P aeruginosa lung disease in patients with CF.}, added-at = {2013-04-12T04:20:05.000+0200}, author = {Hartl, Dominik and Griese, Matthias and Kappler, Matthias and Zissel, Gernot and Reinhardt, Dietrich and Rebhan, Christian and Schendel, Dolores J. and Krauss-Etschmann, Susanne}, biburl = {https://www.bibsonomy.org/bibtex/264debd3c62e6a9f95791512d30219645/aorchid}, doi = {10.1016/j.jaci.2005.09.023}, file = {:allorejection/JAllergyClinImmunol.117.204.pdf:PDF}, groups = {public}, institution = {sity of Munich, Munich, Germany.}, interhash = {608f63d9cc1ab69ad2acfd06fc2affc0}, intrahash = {64debd3c62e6a9f95791512d30219645}, journal = {J Allergy Clin Immunol}, keywords = {pseudomonas mucosal cysticfibrosis pneumonia}, language = {eng}, medline-pst = {ppublish}, month = Jan, number = 1, pages = {204--211}, pii = {S0091-6749(05)02109-3}, pmid = {16387607}, timestamp = {2013-04-12T04:20:05.000+0200}, title = {Pulmonary T(H)2 response in Pseudomonas aeruginosa-infected patients with cystic fibrosis.}, url = {http://dx.doi.org/10.1016/j.jaci.2005.09.023}, username = {aorchid}, volume = 117, year = 2006 }