%0 Journal Article %1 Aust.2013 %A Aust, Gabriela %A Kerner, Christiane %A Gonsior, Susann %A Sittig, Doreen %A Schneider, Hartmut %A Buske, Peter %A Scholz, Markus %A Dietrich, Norman %A Oldenburg, Sindy %A Karpus, Olga N. %A Galle, Jörg %A Amasheh, Salah %A Hamann, Jörg %D 2013 %J Molecular biology of the cell %K Age_Factors Animals Animals,_Newborn Animals,_Suckling/physiology Antigens,_CD55/genetics/metabolism Cell_Proliferation Epithelial_Cells/cytology/metabolism Female Gene_Expression Humans Intestinal_Mucosa/cytology/metabolism Intestine,_Small/cytology/metabolism Membrane_Glycoproteins/genetics/metabolism Mice Mice,_Transgenic Models,_Biological Protein_Binding Protein_Interaction_Domains_and_Motifs Weaning %N 14 %P 2256–2268 %T Mice overexpressing CD97 in intestinal epithelial cells provide a unique model for mammalian postnatal intestinal cylindrical growth %V 24 %X Postnatal enlargement of the mammalian intestine comprises cylindrical and luminal growth, associated with crypt fission and crypt/villus hyperplasia, respectively, which subsequently predominate before and after weaning. The bipartite adhesion G protein-coupled receptor CD97 shows an expression gradient along the crypt-villus axis in the normal human intestine. We here report that transgenic mice overexpressing CD97 in intestinal epithelial cells develop an upper megaintestine. Intestinal enlargement involves an increase in length and diameter but does not affect microscopic morphology, as typical for cylindrical growth. The megaintestine is acquired after birth and before weaning, independent of the genotype of the mother, excluding altered availability of milk constituents as driving factor. CD97 overexpression does not regulate intestinal growth factors, stem cell markers, and Wnt signaling, which contribute to epithelial differentiation and renewal, nor does it affect suckling-to-weaning transition. Consistent with augmented cylindrical growth, suckling but not adult transgenic mice show enlarged crypts and thus more crypt fissions caused by a transient increase of the crypt transit-amplifying zone. Intestinal enlargement by CD97 requires its seven-span transmembrane/cytoplasmic C-terminal fragment but not the N-terminal fragment binding partner CD55. In summary, ectopic expression of CD97 in intestinal epithelial cells provides a unique model for intestinal cylindrical growth occurring in breast-fed infants.

@article{Aust.2013, abstract = {Postnatal enlargement of the mammalian intestine comprises cylindrical and luminal growth, associated with crypt fission and crypt/villus hyperplasia, respectively, which subsequently predominate before and after weaning. The bipartite adhesion G protein-coupled receptor CD97 shows an expression gradient along the crypt-villus axis in the normal human intestine. We here report that transgenic mice overexpressing CD97 in intestinal epithelial cells develop an upper megaintestine. Intestinal enlargement involves an increase in length and diameter but does not affect microscopic morphology, as typical for cylindrical growth. The megaintestine is acquired after birth and before weaning, independent of the genotype of the mother, excluding altered availability of milk constituents as driving factor. CD97 overexpression does not regulate intestinal growth factors, stem cell markers, and Wnt signaling, which contribute to epithelial differentiation and renewal, nor does it affect suckling-to-weaning transition. Consistent with augmented cylindrical growth, suckling but not adult transgenic mice show enlarged crypts and thus more crypt fissions caused by a transient increase of the crypt transit-amplifying zone. Intestinal enlargement by CD97 requires its seven-span transmembrane/cytoplasmic C-terminal fragment but not the N-terminal fragment binding partner CD55. In summary, ectopic expression of CD97 in intestinal epithelial cells provides a unique model for intestinal cylindrical growth occurring in breast-fed infants.}, added-at = {2014-10-13T18:24:39.000+0200}, author = {Aust, Gabriela and Kerner, Christiane and Gonsior, Susann and Sittig, Doreen and Schneider, Hartmut and Buske, Peter and Scholz, Markus and Dietrich, Norman and Oldenburg, Sindy and Karpus, Olga N. and Galle, Jörg and Amasheh, Salah and Hamann, Jörg}, biburl = {https://www.bibsonomy.org/bibtex/214ef467d18d2853e01b1b8d273fdcfcf/drtester}, interhash = {6523d5588eb94abf9fce1f1e217c45fc}, intrahash = {14ef467d18d2853e01b1b8d273fdcfcf}, journal = {Molecular biology of the cell}, keywords = {Age_Factors Animals Animals,_Newborn Animals,_Suckling/physiology Antigens,_CD55/genetics/metabolism Cell_Proliferation Epithelial_Cells/cytology/metabolism Female Gene_Expression Humans Intestinal_Mucosa/cytology/metabolism Intestine,_Small/cytology/metabolism Membrane_Glycoproteins/genetics/metabolism Mice Mice,_Transgenic Models,_Biological Protein_Binding Protein_Interaction_Domains_and_Motifs Weaning}, number = 14, pages = {2256–2268}, timestamp = {2014-10-13T18:24:39.000+0200}, title = {Mice overexpressing CD97 in intestinal epithelial cells provide a unique model for mammalian postnatal intestinal cylindrical growth}, volume = 24, year = 2013 }