%0 Journal Article %1 Cao2014Twostage %A Cao, Limin %A Tang, Xingliang %A Zhang, Xinyuan %A Zhang, Jingtao %A Tian, Xuelei %A Wang, Jingyu %A Xiong, Mingyong %A Xiao, Wei %D 2014 %J Metabolic engineering %K biofuels lignocellulose yeast %R 10.1016/j.ymben.2014.05.001 %T Two-stage transcriptional reprogramming in Saccharomyces cerevisiae for optimizing ethanol production from xylose. %U http://dx.doi.org/10.1016/j.ymben.2014.05.001 %X Conversion of lignocellulosic material to ethanol is a major challenge in second generation bio-fuel production by yeast Saccharomyces cerevisiae. This report describes a novel strategy named "two-stage transcriptional reprogramming (TSTR)" in which key gene expression at both glucose and xylose fermentation phases is optimized in engineered S. cerevisiae. Through a combined genome-wide screening of stage-specific promoters and the balancing of the metabolic flux, ethanol yields and productivity from mixed sugars were significantly improved. In a medium containing 50g/L glucose and 50g/L xylose, the top-performing strain WXY12 rapidly consumed glucose within 12h and within 84h it consistently achieved an ethanol yield of 0.48g/g total sugar, which was 94\% of the theoretical yield. WXY12 utilizes a KGD1 inducible promoter to drive xylose metabolism, resulting in much higher ethanol yield than a reference strain using a strong constitutive PGK1 promoter. These promising results validate the TSTR strategy by synthetically regulating the xylose assimilation pathway towards efficient xylose fermentation. Crown Copyright \copyright 2014. Published by Elsevier Inc. All rights reserved.

@article{Cao2014Twostage, abstract = {Conversion of lignocellulosic material to ethanol is a major challenge in second generation bio-fuel production by yeast Saccharomyces cerevisiae. This report describes a novel strategy named "two-stage transcriptional reprogramming ({TSTR})" in which key gene expression at both glucose and xylose fermentation phases is optimized in engineered S. cerevisiae. Through a combined genome-wide screening of stage-specific promoters and the balancing of the metabolic flux, ethanol yields and productivity from mixed sugars were significantly improved. In a medium containing {50g/L} glucose and {50g/L} xylose, the top-performing strain {WXY12} rapidly consumed glucose within 12h and within 84h it consistently achieved an ethanol yield of 0.48g/g total sugar, which was 94\% of the theoretical yield. {WXY12} utilizes a {KGD1} inducible promoter to drive xylose metabolism, resulting in much higher ethanol yield than a reference strain using a strong constitutive {PGK1} promoter. These promising results validate the {TSTR} strategy by synthetically regulating the xylose assimilation pathway towards efficient xylose fermentation. Crown Copyright {\copyright} 2014. Published by Elsevier Inc. All rights reserved.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Cao, Limin and Tang, Xingliang and Zhang, Xinyuan and Zhang, Jingtao and Tian, Xuelei and Wang, Jingyu and Xiong, Mingyong and Xiao, Wei}, biburl = {https://www.bibsonomy.org/bibtex/2aa0596f68557495cbbbcd94f0250831d/karthikraman}, citeulike-article-id = {13207223}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.ymben.2014.05.001}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/24858789}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=24858789}, day = 21, doi = {10.1016/j.ymben.2014.05.001}, interhash = {70032ec9141cf2a930bfdca9b534cdaf}, intrahash = {aa0596f68557495cbbbcd94f0250831d}, issn = {1096-7184}, journal = {Metabolic engineering}, keywords = {biofuels lignocellulose yeast}, month = may, pmid = {24858789}, posted-at = {2014-06-02 11:20:04}, priority = {2}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {Two-stage transcriptional reprogramming in Saccharomyces cerevisiae for optimizing ethanol production from xylose.}, url = {http://dx.doi.org/10.1016/j.ymben.2014.05.001}, year = 2014 }