%0 Journal Article %1 onwubiko_cross-sectional_2008 %A Onwubiko, Chinwendu %A Swiatlo, Edwin %A McDaniel, Larry S %D 2008 %J Journal of Clinical Microbiology %K Adult, Aged, Agents, Analysis, Bacterial Carrier Conjugate Count, Female, Fingerprinting, Genotype, Humans, Infections, Lymphocyte Male, Microbial Middle Nasopharynx, Pneumococcal Sensitivity Sequence Serotyping, State, Streptococcus Studies, Techniques, Tests, Typing Vaccines, pneumoniae, {Anti-Bacterial} {CD4} {Cross-Sectional} {DNA,} {DNA} {HIV} %N 11 %P 3621--5 %R 10.1128/JCM.01245-08 %T Cross-sectional study of nasopharyngeal carriage of Streptococcus pneumoniae in human immunodeficiency virus-infected adults in the conjugate vaccine era %U http://www.ncbi.nlm.nih.gov/pubmed/18845823 %V 46 %X Human immunodeficiency virus (HIV)-infected patients have an increased rate of pneumococcal infections. Within the HIV-infected population, patients with low CD4(+) cell counts have a higher rate of pneumococcal infection. The purpose of our study was to determine pneumococcal carriage and to examine the serotypes carried by HIV-infected patients after the introduction of the conjugate vaccine. Nasopharyngeal swabs were obtained from patients during routine clinic visits. Samples were cultured on blood agar plates with gentamicin and screened for alpha-hemolysis, optochin sensitivity, and bile solubility. Capsular serotypes were determined by multiplex PCR, multibead assay, or latex agglutination. Antibiotic susceptibility was determined by the Etest method. Multilocus sequence typing was also performed. Of the 175 patients enrolled, 120 patients had absolute CD4(+) cell counts above 200/mm(3) and 55 had counts below 200/mm(3). A total of six (3.4\%) patients carried pneumococci. All but one of these patients had received the 23-valent pneumococcal vaccine within the previous 5 years. Five of the isolates were serotypes that are not included in the 7-valent conjugate vaccine. Immunization with the pneumococcal polysaccharide vaccine does not prevent colonization in HIV-infected patients; however, the observation of carriage of serotypes not included in the conjugate vaccine may be due to herd immunity and serotype replacement effects in the general population.

@article{onwubiko_cross-sectional_2008, abstract = {Human immunodeficiency virus {(HIV)-infected} patients have an increased rate of pneumococcal infections. Within the {HIV-infected} population, patients with low {CD4(+)} cell counts have a higher rate of pneumococcal infection. The purpose of our study was to determine pneumococcal carriage and to examine the serotypes carried by {HIV-infected} patients after the introduction of the conjugate vaccine. Nasopharyngeal swabs were obtained from patients during routine clinic visits. Samples were cultured on blood agar plates with gentamicin and screened for alpha-hemolysis, optochin sensitivity, and bile solubility. Capsular serotypes were determined by multiplex {PCR,} multibead assay, or latex agglutination. Antibiotic susceptibility was determined by the Etest method. Multilocus sequence typing was also performed. Of the 175 patients enrolled, 120 patients had absolute {CD4(+)} cell counts above 200/mm(3) and 55 had counts below 200/mm(3). A total of six (3.4\%) patients carried pneumococci. All but one of these patients had received the 23-valent pneumococcal vaccine within the previous 5 years. Five of the isolates were serotypes that are not included in the 7-valent conjugate vaccine. Immunization with the pneumococcal polysaccharide vaccine does not prevent colonization in {HIV-infected} patients; however, the observation of carriage of serotypes not included in the conjugate vaccine may be due to herd immunity and serotype replacement effects in the general population.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Onwubiko, Chinwendu and Swiatlo, Edwin and {McDaniel}, Larry S}, biburl = {https://www.bibsonomy.org/bibtex/2da51719886a395bed813136bb679df34/jelias}, doi = {10.1128/JCM.01245-08}, interhash = {74481b51e38ef18203fae184b1700a91}, intrahash = {da51719886a395bed813136bb679df34}, issn = {{1098-660X}}, journal = {Journal of Clinical Microbiology}, keywords = {Adult, Aged, Agents, Analysis, Bacterial Carrier Conjugate Count, Female, Fingerprinting, Genotype, Humans, Infections, Lymphocyte Male, Microbial Middle Nasopharynx, Pneumococcal Sensitivity Sequence Serotyping, State, Streptococcus Studies, Techniques, Tests, Typing Vaccines, pneumoniae, {Anti-Bacterial} {CD4} {Cross-Sectional} {DNA,} {DNA} {HIV}}, month = nov, note = {{PMID:} 18845823}, number = 11, pages = {3621--5}, timestamp = {2011-03-11T10:06:16.000+0100}, title = {Cross-sectional study of nasopharyngeal carriage of Streptococcus pneumoniae in human immunodeficiency virus-infected adults in the conjugate vaccine era}, url = {http://www.ncbi.nlm.nih.gov/pubmed/18845823}, volume = 46, year = 2008 }