%0 Journal Article %1 snyder_comparison_2010 %A Snyder, James W %A Munier, Gina K %A Johnson, Charles L %D 2010 %J Journal of Clinical Microbiology %K imported %R 10.1128/JCM.01326-09 %T Comparison of the BD GeneOhm Methicillin-Resistant Staphylococcus aureus (MRSA) PCR assay to Culture using BBL CHROMagar MRSA for the Detection of MRSA in Nasal Surveillance Cultures from ICU Patients %U http://www.ncbi.nlm.nih.gov/pubmed/20181916 %X This study compared the BD GeneOhm(TM) MRSA real-time PCR assay to BBL(TM) CHROMagar(TM) MRSA for the detection of methicillin-resistant Staphylococcus aureus in 627 nasal surveillance specimens collected from ICU-patients. The PCR assay had sensitivity, specificity, positive predictive value and negative predictive values of 100\%, 96.7\%, 70.3\%, and 100\%, respectively. Nine of nineteen false-positive PCR specimens grew methicillin-susceptible S. aureus (MSSA) from broth enrichment culture of which two demonstrated evidence of mecA gene dropout. Compared to BBL(TM) CHROMagar(TM) MRSA, the BD GeneOhm(TM) MRSA PCR assay demonstrated sensitivity and specificity above 95\% for the detection of MRSA nasal colonization compared to chromogenic agar and provided shorter turn around time in generating positive and negative final results.

@article{snyder_comparison_2010, abstract = {This study compared the {BD} {GeneOhm(TM)} {MRSA} real-time {PCR} assay to {BBL(TM)} {CHROMagar(TM)} {MRSA} for the detection of methicillin-resistant Staphylococcus aureus in 627 nasal surveillance specimens collected from {ICU-patients.} The {PCR} assay had sensitivity, specificity, positive predictive value and negative predictive values of 100\%, 96.7\%, 70.3\%, and 100\%, respectively. Nine of nineteen false-positive {PCR} specimens grew methicillin-susceptible S. aureus {(MSSA)} from broth enrichment culture of which two demonstrated evidence of {mecA} gene dropout. Compared to {BBL(TM)} {CHROMagar(TM)} {MRSA,} the {BD} {GeneOhm(TM)} {MRSA} {PCR} assay demonstrated sensitivity and specificity above 95\% for the detection of {MRSA} nasal colonization compared to chromogenic agar and provided shorter turn around time in generating positive and negative final results.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Snyder, James W and Munier, Gina K and Johnson, Charles L}, biburl = {https://www.bibsonomy.org/bibtex/29833015b65063f6c42727ab049b90efd/jelias}, doi = {10.1128/JCM.01326-09}, interhash = {77ffd85cb7b45c5111c253b922a8cbe2}, intrahash = {9833015b65063f6c42727ab049b90efd}, issn = {{1098-660X}}, journal = {Journal of Clinical Microbiology}, keywords = {imported}, month = feb, note = {{PMID:} 20181916}, timestamp = {2011-03-11T10:06:18.000+0100}, title = {Comparison of the {BD} {GeneOhm} {Methicillin-Resistant} Staphylococcus aureus {(MRSA)} {PCR} assay to Culture using {BBL} {CHROMagar} {MRSA} for the Detection of {MRSA} in Nasal Surveillance Cultures from {ICU} Patients}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20181916}, year = 2010 }