%0 Journal Article %1 wang_antimicrobial_2006 %A Wang, Bei %A shui Xu, Jin %A xian Wang, Chang %A huang Mi, Zu %A pu Pu, Yue %A Hui, Mamie %A Ling, Thomas K W %A Chan, Chiu-Yeung %D 2006 %J Journal of Medical Microbiology %K Acid Adult, Agents, Amino Analysis, Bacterial, China, Drug Female, Fluoroquinolones, Gonorrhea, Gyrase, Humans, Male, Microbial Missense, Mutation, Neisseria Resistance, Sensitivity Sequence Substitution, Tests, Topoisomerase gonorrhoeae, {Anti-Bacterial} {DNA,} {DNA} {IV,} %N Pt 9 %P 1251--1255 %R 10.1099/jmm.0.46401-0 %T Antimicrobial susceptibility of Neisseria gonorrhoeae isolated in Jiangsu Province, China, with a focus on fluoroquinolone resistance %U http://www.ncbi.nlm.nih.gov/pubmed/16914656 %V 55 %X In this study, the phenotypic and genotypic resistance to fluoroquinolones in Neisseria gonorrhoeae isolated in Jiangsu Province, China, was analysed. In vitro susceptibility testing of eight antimicrobial agents, including ciprofloxacin and levofloxacin, against 95 clinical isolates was carried out. Detection of mutations in the gyrA and parC genes was performed by sequence analysis. The clinical isolates demonstrated 100\% resistance to ciprofloxacin and 98.9\% non-susceptibility to levofloxacin. All of the isolates were susceptible to cefotaxime and ceftriaxone. For cefepime, spectinomycin and tetracycline, 98.9, 94.7 and 1.1\% of the isolates were susceptible, respectively. None of the isolates was susceptible to penicillin. Five types based on gyrA mutations could be categorized among 54 isolates with seven different mutation sites found on their parC gene. Analysis of sequence results showed that the gyrA mutation Asp-95--\textgreaterAla and the parC mutations Ser-87--\textgreaterArg and Ser-87--\textgreaterAsn made a significant contribution to the resistance to fluoroquinolones, in addition to double mutations found in each gene. Therefore, the use of fluoroquinolones in the treatment of N. gonorrhoeae infections in Jiangsu Province is not recommended, while the use of third- and fourth-generation cephalosporins and spectinomycin is recommended.

@article{wang_antimicrobial_2006, abstract = {In this study, the phenotypic and genotypic resistance to fluoroquinolones in Neisseria gonorrhoeae isolated in Jiangsu Province, China, was analysed. In vitro susceptibility testing of eight antimicrobial agents, including ciprofloxacin and levofloxacin, against 95 clinical isolates was carried out. Detection of mutations in the {gyrA} and {parC} genes was performed by sequence analysis. The clinical isolates demonstrated 100\% resistance to ciprofloxacin and 98.9\% non-susceptibility to levofloxacin. All of the isolates were susceptible to cefotaxime and ceftriaxone. For cefepime, spectinomycin and tetracycline, 98.9, 94.7 and 1.1\% of the isolates were susceptible, respectively. None of the isolates was susceptible to penicillin. Five types based on {gyrA} mutations could be categorized among 54 isolates with seven different mutation sites found on their {parC} gene. Analysis of sequence results showed that the {gyrA} mutation {Asp-95--{\textgreater}Ala} and the {parC} mutations {Ser-87--{\textgreater}Arg} and {Ser-87--{\textgreater}Asn} made a significant contribution to the resistance to fluoroquinolones, in addition to double mutations found in each gene. Therefore, the use of fluoroquinolones in the treatment of N. gonorrhoeae infections in Jiangsu Province is not recommended, while the use of third- and fourth-generation cephalosporins and spectinomycin is recommended.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Wang, Bei and shui Xu, Jin and xian Wang, Chang and huang Mi, Zu and pu Pu, Yue and Hui, Mamie and Ling, Thomas K W and Chan, {Chiu-Yeung}}, biburl = {https://www.bibsonomy.org/bibtex/224179ef748c6a49b9f155f03ebf6f205/jelias}, doi = {10.1099/jmm.0.46401-0}, interhash = {7812ec22a6373fb9a2ec97e36c284fc3}, intrahash = {24179ef748c6a49b9f155f03ebf6f205}, issn = {0022-2615}, journal = {Journal of Medical Microbiology}, keywords = {Acid Adult, Agents, Amino Analysis, Bacterial, China, Drug Female, Fluoroquinolones, Gonorrhea, Gyrase, Humans, Male, Microbial Missense, Mutation, Neisseria Resistance, Sensitivity Sequence Substitution, Tests, Topoisomerase gonorrhoeae, {Anti-Bacterial} {DNA,} {DNA} {IV,}}, month = sep, note = {{PMID:} 16914656}, number = {Pt 9}, pages = {1251--1255}, timestamp = {2011-03-11T10:06:41.000+0100}, title = {Antimicrobial susceptibility of Neisseria gonorrhoeae isolated in Jiangsu Province, China, with a focus on fluoroquinolone resistance}, url = {http://www.ncbi.nlm.nih.gov/pubmed/16914656}, volume = 55, year = 2006 }