%0 Journal Article %1 lin_1h-13c_2010 %A Lin, Eugene C %A Wu, Chin H %A Yang, Yuan %A Grant, Christopher V %A Opella, Stanley J %D 2010 %J J. Magn. Reson. %K Bacteriophages,Carbon Fields,Hydrogen,Indicators Isotopes,Peptides,Proteins,Spin Labeling,Magnetic Labels Radioisotopes,Electromagnetic Reagents,Isotope Resonance Spectroscopy,Nitrogen and %N 1 %P 105--111 %R 10.1016/j.jmr.2010.06.011 %T 1H-13C separated local field spectroscopy of uniformly 13C labeled peptides and proteins %V 206 %X By incorporating homonuclear decoupling on both the (1)H and (13)C channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100\% labeled with (13)C. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated Observation) can be performed as a conventional two-dimensional experiment, or with windowed detection as a one-dimensional experiment that offers flexibility as a building block for shiftless and other multidimensional triple-resonance experiments with the inclusion of (15)N irradiation. The triple-resonance MAGC probe used to perform these experiments at 500 MHz is described.

@article{lin_1h-13c_2010, abstract = {By incorporating homonuclear decoupling on both the (1)H and (13)C channels it is feasible to obtain high-resolution two-dimensional separated local field spectra of peptides and proteins that are 100{\%} labeled with (13)C. Dual-PISEMO (Polarization Inversion Spin Exchange Modulated Observation) can be performed as a conventional two-dimensional experiment, or with windowed detection as a one-dimensional experiment that offers flexibility as a building block for shiftless and other multidimensional triple-resonance experiments with the inclusion of (15)N irradiation. The triple-resonance MAGC probe used to perform these experiments at 500 MHz is described.}, added-at = {2017-03-14T02:48:56.000+0100}, author = {Lin, Eugene C and Wu, Chin H and Yang, Yuan and Grant, Christopher V and Opella, Stanley J}, biburl = {https://www.bibsonomy.org/bibtex/28f6ee7b9d5a0e14797df7be9b7df2180/nmrresource}, doi = {10.1016/j.jmr.2010.06.011}, interhash = {7dedd7bc58d1d28610b446dbad221ac3}, intrahash = {8f6ee7b9d5a0e14797df7be9b7df2180}, issn = {1096-0856}, journal = {J. Magn. Reson.}, keywords = {Bacteriophages,Carbon Fields,Hydrogen,Indicators Isotopes,Peptides,Proteins,Spin Labeling,Magnetic Labels Radioisotopes,Electromagnetic Reagents,Isotope Resonance Spectroscopy,Nitrogen and}, month = sep, number = 1, pages = {105--111}, pmid = {20637662}, timestamp = {2017-03-14T02:49:21.000+0100}, title = {{1H-13C separated local field spectroscopy of uniformly 13C labeled peptides and proteins}}, volume = 206, year = 2010 }