%0 Journal Article %1 phagesim %A Kindt, J %A Tzlil, S %A Ben-Shaul, A %A Gelbart, W M %D 2001 %J Proc Natl Acad Sci U S A %K phage %N 24 %P 13671-13674 %R 10.1073/pnas.241486298 %T DNA packaging and ejection forces in bacteriophage %U http://www.ncbi.nlm.nih.gov/pubmed/11707588 %V 98 %X We calculate the forces required to package (or, equivalently, acting to eject) DNA into (from) a bacteriophage capsid, as a function of the loaded (ejected) length, under conditions for which the DNA is either self-repelling or self-attracting. Through computer simulation and analytical theory, we find the loading force to increase more than 10-fold (to tens of piconewtons) during the final third of the loading process; correspondingly, the internal pressure drops 10-fold to a few atmospheres (matching the osmotic pressure in the cell) upon ejection of just a small fraction of the phage genome. We also determine an evolution of the arrangement of packaged DNA from toroidal to spool-like structures.

@article{phagesim, abstract = {We calculate the forces required to package (or, equivalently, acting to eject) DNA into (from) a bacteriophage capsid, as a function of the loaded (ejected) length, under conditions for which the DNA is either self-repelling or self-attracting. Through computer simulation and analytical theory, we find the loading force to increase more than 10-fold (to tens of piconewtons) during the final third of the loading process; correspondingly, the internal pressure drops 10-fold to a few atmospheres (matching the osmotic pressure in the cell) upon ejection of just a small fraction of the phage genome. We also determine an evolution of the arrangement of packaged DNA from toroidal to spool-like structures.}, added-at = {2014-02-24T11:42:44.000+0100}, author = {Kindt, J and Tzlil, S and Ben-Shaul, A and Gelbart, W M}, biburl = {https://www.bibsonomy.org/bibtex/239d4e9d9f07bd03b08cc59d8e4b17b90/ross_mck}, description = {DNA packaging and ejection forces i... [Proc Natl Acad Sci U S A. 2001] - PubMed - NCBI}, doi = {10.1073/pnas.241486298}, interhash = {86e148c52e7f89c3364160bd106d10d0}, intrahash = {39d4e9d9f07bd03b08cc59d8e4b17b90}, journal = {Proc Natl Acad Sci U S A}, keywords = {phage}, month = nov, number = 24, pages = {13671-13674}, pmid = {11707588}, timestamp = {2014-02-24T11:42:44.000+0100}, title = {DNA packaging and ejection forces in bacteriophage}, url = {http://www.ncbi.nlm.nih.gov/pubmed/11707588}, volume = 98, year = 2001 }