%0 Journal Article %1 citeulike:636694 %A Dong, H %A Li, C M M. %A Zhou, Q %A Sun, J B B. %A Miao, J M M. %C School of Chemical and Biomedical Engineering, Nanyang Technological University, Nanyang Avenue, Singapore 639798, Singapore. %D 2006 %J Biosens Bioelectron %K electrochemistry immunoelectrode %R 10.1016/j.bios.2006.01.034 %T Sensitive electrochemical enzyme immunoassay microdevice based on architecture of dual ring electrodes with a sensing cavity chamber. %U http://dx.doi.org/10.1016/j.bios.2006.01.034 %X A novel electrochemical detection architecture was investigated for enzyme immunoassay sensors. Microchips with dual-ring working and counter electrodes, and a sensing cavity chamber were made on glass slides. The glass surface of the microchip was coated by 3-aminopropyltriethoxysilane (APTES). Goat IgG, as a example, was covalently captured on APTES-modified glass surfaces through glutaraldehyde (GA) as a cross-linker. Enzyme substrate, p-aminophenyl phosphate (PAPP) was prepared by electrolysis. The enzyme conversion from home-synthetic PAPP to p-aminophenol (PAP) was examined by differential pulse voltammetry (DPV). A competitive inhibition enzyme-linked immunosorbant assay (ELISA) was designed to test the system. Experimental results demonstrate that a detection limit of 118fg/ml of goat IgG and a dynamic range of 118fg/ml to 1.18ng/ml, up to five orders of magnitude could be achieved. Due to its novel architecture design and electronic detection scheme, the method can be used to fabricate portable electrochemical ELISA lab-on-chip systems. The technology could have great potential in clinical diagnostic applications.

@article{citeulike:636694, abstract = {A novel electrochemical detection architecture was investigated for enzyme immunoassay sensors. Microchips with dual-ring working and counter electrodes, and a sensing cavity chamber were made on glass slides. The glass surface of the microchip was coated by 3-aminopropyltriethoxysilane (APTES). Goat IgG, as a example, was covalently captured on APTES-modified glass surfaces through glutaraldehyde (GA) as a cross-linker. Enzyme substrate, p-aminophenyl phosphate (PAPP) was prepared by electrolysis. The enzyme conversion from home-synthetic PAPP to p-aminophenol (PAP) was examined by differential pulse voltammetry (DPV). A competitive inhibition enzyme-linked immunosorbant assay (ELISA) was designed to test the system. Experimental results demonstrate that a detection limit of 118fg/ml of goat IgG and a dynamic range of 118fg/ml to 1.18ng/ml, up to five orders of magnitude could be achieved. Due to its novel architecture design and electronic detection scheme, the method can be used to fabricate portable electrochemical ELISA lab-on-chip systems. The technology could have great potential in clinical diagnostic applications.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {School of Chemical and Biomedical Engineering, Nanyang Technological University, Nanyang Avenue, Singapore 639798, Singapore.}, author = {Dong, H and Li, C M M. and Zhou, Q and Sun, J B B. and Miao, J M M.}, biburl = {https://www.bibsonomy.org/bibtex/29260cdf67ce93cd7196790b654b38717/biblio24}, citeulike-article-id = {636694}, doi = {10.1016/j.bios.2006.01.034}, interhash = {87fbd8dd750c24b5e9138803c74709e6}, intrahash = {9260cdf67ce93cd7196790b654b38717}, issn = {0956-5663}, journal = {Biosens Bioelectron}, keywords = {electrochemistry immunoelectrode}, month = {March}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Sensitive electrochemical enzyme immunoassay microdevice based on architecture of dual ring electrodes with a sensing cavity chamber.}, url = {http://dx.doi.org/10.1016/j.bios.2006.01.034}, year = 2006 }