%0 Journal Article %1 Facette2001 %A Facette, M. R. %A Mccully, M. E. %A Shane, M. W. %A Canny, M. J. %D 2001 %J Plant Physiology and Biochemistry %K bibtex-import citeulikeExport %P 59--66 %T Measurements of the time to refill embolised vessels %V 39

@article{Facette2001, added-at = {2019-03-31T01:14:40.000+0100}, author = {Facette, M. R. and Mccully, M. E. and Shane, M. W. and Canny, M. J.}, biburl = {https://www.bibsonomy.org/bibtex/209b08fe6929f73d4f05884c64f35c56d/dianella}, citeulike-article-id = {1523685}, comment = {(private-note)An embolized vessel is not necessarily filled with gas from one end to the other. All our observations show individual vessels with varying proportions of gas and liquid (figure 1C; figures 5 and 6 of Canny [3, 4]; figure 1i?k of McCully et al. [13]; figure 3C?G of Buchard et al. [2]; figure 2F and G of Shane and McCully [17]; figures 16?19 of Berndt et al. [1]; figure 5B of Tyree et al. [18]). From this viewpoint, the characterization of a unique ?time to refill? for the vessels of even a single root is meaningless. (they're all different amounts of fullness already) so they fit an exponential curve (fit really well) and then calculated a half-time for refilling (why they didn't just measure the time when 50\% of vessels were full. The composition of the gas in the embolised vessel influences refilling time. (and also the gas-concentration in the surrounding water) The pressure changes in the gas phase after cavitation have been recorded many times by Zimmermann and his colleagues using the xylem-pressure probe (e.g. figures 1 and 2 of Zimmermann et al. [20]; and recently by Wei et al. [19]). THey record the transition from water vapor to air in cavitated vessels (how?) Lewis et al. also did. Because there is currently no technique available to follow changes in embolisms in real time in intact, transpiring organs, the present study of vessel refilling has been done by destructive sampling of roots disconnected from the transpiration stream. hahaha cryo-technique also Huang C.X., Canny M.J., Oates K., McCully M.E., Planing frozen hydrated plant specimens for SEM observation and EDX microanalysis, Microsc. Res. Tech. 28 (1994) 67?74. 14] McCully M.E., Shane M.W., Baker A.N., Huang C.X., Ling L.E.C., Canny M.J., The reliability of cryoSEM for the observation and quantification of xylem embolisms and quantitative analysis of xylem sap in situ, J. Microsc. 198 (2000) 24?33. Details of the methods of preparation and examination of the frozen specimens were described previously [10, 14]. Briefly, the central part of each root piece was trimmed under LN2, mounted on a stub and planed in a cryo-microtome at ?80 C to give a smooth transverse face. The specimen was lightly etched in the column of the microscope to reveal cell outlines, coated with aluminium, and observed in secondary electron mode at 7 kV. Embolized vessels are easily distinguished from those that are sap-filled (figure 1). The gas-filled vessels appear either as empty tubes or partly filled with debris which was pushed into them during the planing of the frozen block. The contents of vessels was assessed either directly on the screen or on micrographs. A vessel was considered embolized if it contained any gas space in the transverse profile. Only the large late metaxylem vessels were considered, because they are the ones which consistently show the most embolism. They are referred to here as ?vessels?.}, interhash = {8a0d893f0191a0338b19c47746e8e597}, intrahash = {09b08fe6929f73d4f05884c64f35c56d}, journal = {Plant Physiology and Biochemistry}, keywords = {bibtex-import citeulikeExport}, pages = {59--66}, posted-at = {2007-07-31 06:17:28}, priority = {2}, timestamp = {2019-03-31T01:16:26.000+0100}, title = {{Measurements of the time to refill embolised vessels}}, volume = 39, year = 2001 }