%0 Journal Article %1 Rueger2010 %A Rueger, S. %A Ehrenberger, W. %A Arend, M. %A Gessner, P. %A Zimmermann, G. %A Zimmermann, D. %A Bentrup, F. W. %A Nadler, A. %A Raveh, E. %A Sukhorukov, V. L. %A Zimmermann, U. %C PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS %D 2010 %I ELSEVIER SCIENCE BV %J AGRICULTURAL WATER MANAGEMENT %K vladimir %N 2 %P 283-290 %T Comparative monitoring of temporal and spatial changes in tree water status using the non-invasive leaf patch clamp pressure probe and the pressure bomb %U http://dx.doi.org/10.1016/j.agwat.2010.08.022 %V 98 %X Real-time monitoring of plant water status under field conditions remains difficult to quantify. Here we give evidence that the magnetic-based leaf patch clamp pressure (LPCP) probe is a non-invasive and online-measuring method that can elucidate short- and long-term temporal and spatial dynamics of leaf water status of trees with high precision in real time. Measurements were controlled remotely by telemetry and data transfer to the Internet. Concomitant measurements using the pressure chamber technique (frequently applied for leaf water status monitoring) showed that both techniques yield in principle the same results despite of the high sampling variability of the pressure chamber data. There was a very good correlation between the output pressure signals of the LPCP probe and the balancing pressure values (on average r(2) = 0.90 +/- 0.05; n = 8), i.e. the external pressure at which water appears at the cut end of a leaf under pressure chamber conditions. Simultaneously performed direct measurements of leaf cell turgor pressure using the well-established cell turgor pressure probe technique evidenced that both techniques measure relative changes in leaf turgor pressure. The output pressure signals of the LPCP probe and the balancing pressure values were inversely correlated to turgor pressure. Consistent with this, the balancing pressure values and the cell turgor pressure values could be fitted quite well by the same firm theoretical backing derived recently for the LPCP probe (Zimmermann et al., 2008). This finding suggests that use of the LPCP probe technique in agricultural water management can be built up on the knowledge accumulated on spot leaf or stem water potential measurements. (C) 2010 Elsevier B.V. All rights reserved.

@article{Rueger2010, abstract = {Real-time monitoring of plant water status under field conditions remains difficult to quantify. Here we give evidence that the magnetic-based leaf patch clamp pressure (LPCP) probe is a non-invasive and online-measuring method that can elucidate short- and long-term temporal and spatial dynamics of leaf water status of trees with high precision in real time. Measurements were controlled remotely by telemetry and data transfer to the Internet. Concomitant measurements using the pressure chamber technique (frequently applied for leaf water status monitoring) showed that both techniques yield in principle the same results despite of the high sampling variability of the pressure chamber data. There was a very good correlation between the output pressure signals of the LPCP probe and the balancing pressure values (on average r(2) = 0.90 +/- 0.05; n = 8), i.e. the external pressure at which water appears at the cut end of a leaf under pressure chamber conditions. Simultaneously performed direct measurements of leaf cell turgor pressure using the well-established cell turgor pressure probe technique evidenced that both techniques measure relative changes in leaf turgor pressure. The output pressure signals of the LPCP probe and the balancing pressure values were inversely correlated to turgor pressure. Consistent with this, the balancing pressure values and the cell turgor pressure values could be fitted quite well by the same firm theoretical backing derived recently for the LPCP probe (Zimmermann et al., 2008). This finding suggests that use of the LPCP probe technique in agricultural water management can be built up on the knowledge accumulated on spot leaf or stem water potential measurements. (C) 2010 Elsevier B.V. All rights reserved.}, added-at = {2011-03-02T09:35:06.000+0100}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, affiliation = {Zimmermann, U (Reprint Author), Univ Wurzburg, Lehrstuhl Biotechnol, Biozentrum, D-97074 Wurzburg, Germany. {[}Rueger, S.; Ehrenberger, W.; Gessner, P.; Zimmermann, G.; Sukhorukov, V. L.; Zimmermann, U.] Univ Wurzburg, Lehrstuhl Biotechnol, Biozentrum, D-97074 Wurzburg, Germany. {[}Arend, M.] Eidg Forsch Anstalt Wald Schnee \& Landschaft, WSL, CH-8903 Birmensdorf, Switzerland. {[}Zimmermann, D.] Max Planck Inst Biophys, Biophys Chem Abt, D-60439 Frankfurt, Germany. {[}Bentrup, F. -W.] Salzburg Univ, Abt Pflanzenphysiol, A-5020 Salzburg, Austria. {[}Nadler, A.] ARO Volcani Ctr, Inst Soil Water \& Environm Sci, IL-50250 Bet Dagan, Israel. {[}Raveh, E.] ARO Volcani Ctr, Gilat Res Ctr, IL-85280 Negev, Israel.}, author = {Rueger, S. and Ehrenberger, W. and Arend, M. and Gessner, P. and Zimmermann, G. and Zimmermann, D. and Bentrup, F. W. and Nadler, A. and Raveh, E. and Sukhorukov, V. L. and Zimmermann, U.}, author-email = {zimmermann@biozentrum.uni-wuerzburg.de}, biburl = {https://www.bibsonomy.org/bibtex/21c69bec3886a2ac3d60b7044ecc0a144/reichert}, doc-delivery-number = {696PB}, funding-acknowledgement = {AIF {[}KF 0054703WM8]}, funding-text = {This work was supported by a grant from the AIF (no. KF 0054703WM8) to U. Z. We would like to thank Ewa Stepien-Botsch for her great help in evaluation of the LPCP datasets and Zipora Hill-man Bronshtain for her great organisation, support and control of the field experiments at Gilat, Israel.}, groups = {public}, interhash = {8be55f3fe4042dbe8e7d8106aeb0ac30}, intrahash = {1c69bec3886a2ac3d60b7044ecc0a144}, issn = {0378-3774}, journal = {AGRICULTURAL WATER MANAGEMENT}, journal-iso = {Agric. Water Manage.}, keywords = {vladimir}, keywords-plus = {STEM RADIUS CHANGES; SAP FLOW; HEAT-PULSE; TURGOR PRESSURE; ORANGE TREES; FRUIT SIZE; CROP LOAD; IRRIGATION; PLANTS; STRESS}, language = {English}, month = {DEC 15}, number = 2, number-of-cited-references = {27}, pages = {283-290}, publisher = {ELSEVIER SCIENCE BV}, subject-category = {Agronomy; Water Resources}, times-cited = {0}, timestamp = {2011-03-09T14:16:20.000+0100}, title = {Comparative monitoring of temporal and spatial changes in tree water status using the non-invasive leaf patch clamp pressure probe and the pressure bomb}, type = {Article}, unique-id = {ISI:000285452200007}, url = {http://dx.doi.org/10.1016/j.agwat.2010.08.022}, username = {reichert}, volume = 98, year = 2010 }